| 去甲二氢愈创木酸部分抑制大鼠局灶性脑缺血后炎症反应 |
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| 引用本文： | 储利胜,方三华,周宇,印媛君,柯庆,陈伟燕,魏尔清.去甲二氢愈创木酸部分抑制大鼠局灶性脑缺血后炎症反应[J].生理学报,2010(2). |
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| 作者姓名： | 储利胜 方三华 周宇 印媛君 柯庆 陈伟燕 魏尔清 |
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| 作者单位： | 浙江中医药大学生理学教研室;浙江大学医学部药理系; |
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| 基金项目： | supported by the National Natural Science Foundation of China (No. 30371637); the Scientific and Technological Program of Medicine and Health of Zhejiang Province, China (No. 2004A061) |
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| 摘 要： | 本实验旨在观察去甲二氢愈创木酸(nordihydroguaiaretic acid,NDGA)对大鼠局灶性脑缺血后炎症细胞聚集的作用及其机制。在大鼠大脑中动脉阻塞30min后进行再灌注72h,在再灌注30min,2、24、48h时分别腹腔注射一次NDGA(5、10mg/kg)。再灌注72h后检测脑损伤、内源性IgG渗出、中性粒细胞和巨噬细胞/小胶质细胞聚集、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)mRNA和蛋白表达,并在再灌注3h后检测脑内5-脂氧酶(5-lipoxygenase,5-LOX)的催化产物白三烯B4(leukotriene B4,LTB4)和半胱氨酰白三烯(cysteinyl leukotrienes,CysLTs)含量。结果显示:NDGA能显著改善脑损伤,减少内源性IgG渗出、中性粒细胞浸润、ICAM-1mRNA和蛋白表达,同时降低脑内LTB4和CysLTs含量,但对巨噬细胞/小胶质细胞聚集没有影响。上述结果提示,NDGA对脑缺血亚急性期炎症反应的抑制主要表现为减少中性粒细胞浸润,机制可能与抑制5-LOX激活有关。
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| 关 键 词： | 去甲二氢愈创木酸 脑缺血 中性粒细胞 巨噬细胞 小胶质细胞 5-脂氧酶 |
Nordihydroguaiaretic acid partially inhibits inflammatory responses after focal cerebral ischemia in rats |
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| CHU Li-Sheng,FANG San-Hua,ZHOU Yu,YIN Yuan-Jun,KE Qing,CHEN Wei-Yan,WEI Er-Qing.Nordihydroguaiaretic acid partially inhibits inflammatory responses after focal cerebral ischemia in rats[J].Acta Physiologica Sinica,2010(2). |
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| Authors: | CHU Li-Sheng FANG San-Hua ZHOU Yu YIN Yuan-Jun KE Qing CHEN Wei-Yan WEI Er-Qing |
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| Institution: | CHU Li-Sheng1,FANG San-Hua2,ZHOU Yu2,YIN Yuan-Jun1,KE Qing1,CHEN Wei-Yan1,WEI Er-Qing2 1Department of Physiology,Zhejiang Chinese Medical University,Hangzhou 310053,China,2Department of Pharmacology,School of Medicine,Zhejiang University,Hangzhou 310058 |
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| Abstract: | The aim of the present study is to investigate the role of nordihydroguaiaretic acid (NDGA) on inflammatory cells accumu- lation after focal cerebral ischemia and the underlying mechanism.Focal cerebral ischemia was induced by 30 min of middle cerebral artery occlusion (MCAO) followed by 72 h of reperfusion.NDGA (5 and 10 mg/kg) was administered intraperitoneally 30 min,2,24,48 h after reperfusion,respectively.The brain injuries were observed by neurological and histological examination.Endogenous IgG exuda... |
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| Keywords: | nordihydroguaiaretic acid brain ischemia neutrophils macrophages microglia 5-lipoxygenase |
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