四氯化碳中毒对大鼠离体再生肝细胞钾离子外漏的影响

本工作用三种剂量四氯化碳(CCl_4,10,15和20mmol/L)损伤正常大鼠离体肝细胞,分别在5,10,15和20min测定细胞内K~+和GPT漏出量。实验观察到细胞内K~+和GPT漏出量与CCl_4染毒的剂量和时间有明显关系,而且K~+漏出量较GPT更能灵敏地反映细胞的损伤程度;用中等剂量CCl_4(15mmol/L)损伤离体再生肝细胞20min后,细胞内K~+漏出的变化百分数明显低于正常肝细胞。这些结果表明,大鼠离体再生肝细胞具有较强的抗CCl_4损伤作用,其机制可能与再生肝细胞膜稳定性较强有关。

EFFECT OF CARBON TETRACHLORIDE INTOXICATION ON LEAKAGE OF INTRACELLULAR POTASSIUM IN REGENERATING RAT HEPATOCYTES IN VITRO

Leakage of intracellular potassium and GPT in normal rat hepatocytes exposedto 10, 15 and 20 mmol / L of carbon tetrachloride (CCl_4) in vitro was determinedat 5, 10, 15 and 20 min after cell intoxication. Both of the intracellular potassiumand GPT were clearly dose- and time-dependent with CCl_4, but the leakage of in-tracellular potassium is a more sensitive index to indicate ccll injury than the later.The percent change of intracellular potassium in regenerating rat hepatocytes 20min after CCl_4 (15 mmol/L) intoxication was markedly reduced than that in nor-mal rat. This finding indicates that regenerating hepatocytes are resistant to CCl_4hepatotoxicity in vitro, and its mechanism presumably is due to a higher stabilityof regenerating liver cell membrane.