热休克蛋白、蛋白水解酶和磷酸酶在大鼠肝再生中的含量和活性变化

本文以２／３肝切除（ｐａｒｔｉａｌ ｈｅｐａｔｅｃｔｏｍｙ，ＰＨ）大鼠为模型，探讨了ＰＨ后酸性和碱性磷酸酶（ａｃｉｄ ａｎｄ ａｌｋａｌｉｎｅ ｐｈｏｓｐｈａｔａｓｅｓ，ＡＣＰ和ＡＫＰ），构成性热休克蛋白７０／诱导性热休克蛋白６８（ＨＳＣ７／ＨＳＰ６８），酸性和中性蛋白水解酶在肝再生期间（０－１４４ｈ）的动态变化。结果显示，在肝切除后的肝再生期间；（１）ＡＣＰ和ＡＫＰ均出现两个活性高峰（４和４８ｈ

Changes in the content and activity of HSC70/HSP68, proteinases and phosphatases during liver regeneration

This article reports changes in acid and alkaline phosphatases (ACP and AKP),conshtutive heat shock protein 70/induced heat shock protein 68 (HSC70/HSP68) andacid and neutal proteinases ddring liver regeneration (0 ~ 144 h) after 2/3 hepatectomy(pwhal hepatectomy, PH). Both ACP and AKP had two achve peaks at 4 and 48 h, 16and 96 h, respectively, which were followed by sighficant decrease. The content ofHSC70/HSP68 also showed two Peaks (16 and 96 h), of which the content after thesecond Peak decnaed more obviously than after the first peak. Moreover a 90 kD neutalproteinase was induced at the time from 2 to 6 h and a 27 kD acid proteinase was inducedat 36 h. The results suggest that the ACP and the 90 kD neuhal proteinase rnayparticipate in activahng hepatocytes from G0-Phase into G1-phase, and are and HSC70/HSP68 may play a role ndnly in DNA synthesis, cellular metabolism and proliferation.Fdriermore 27 kD acid proteinase may be involved in re-differentiation of hepatocytesand reconsmichon of liver tissue.