雌激素在兔胚泡着床中的作用——子宫雌激素受体的研究

为了验证雌激素作用存在于完整的和去卵巢兔胚泡着床过程中,本文用~3H—雌二醇交换法测定了早期妊娠 D_3、D_5、D_7子宫组织的雌二醇受体浓度,并与去卵巢后仅补充孕酮的 D_7子宫作比较。结果显示:在 D_3、D_5、D_7的子宫细胞浆和细胞核中都存在雌二醇受体。妊娠7天去卵巢兔的子宫细胞核中雌二醇受体量与妊娠7天卵巢完整的兔作比较,二者无明显差异。这说明去卵巢后子宫细胞核中雌激素受体浓度并未受影响,因此雌激素作用依然存在。关于胚泡是否有雌激素受体的问题,本工作在胚龄5、6、7天胚泡中未测得雌二醇受体。据此,胚泡着床时,雌激素的作用可能主要通过母体子宫组织而发挥其生理效应。

ROLE OF OESTROGEN AT IMPLANTATION STAGE IN THE RABBIT UTERUS WITH SPECIAL REFERENCE TO UTERINE OESTRADIOL RECEPTORS

For identification of the existence of oestrogen effect during blastocyst implan-tation,the amount of oestradiol receptors in the uteri of intact and ovariectomizedearly pregnant rabbits was determined with ~3H-oestradiol-exchange technique.The results indicated that oestrogen receptor was found in the cytoplasm and nucleiof the uterine tissues on Day 3,5 and 7 of pregnancy.No marked difference wasfound in the concentration of oestradiol receptor in the Day 7 pregnant uterusnuclei between the intact and the ovarectomized rabbits.That the amount ofoestrogen receptors in nuclei was unaffected after ovariectomy provided a strongevidence of the existence of an oestrogen effect in the process of blastocyst implanta-tion.The same method was used to detect whether there were oestradiol recep-tors in blastocysts.The result showed that no oestradiol receptors could be foundin the Day 5,6 and 7 blastoeysts.This suggests that the effect of oestrogen onthe implantation of blastocysts is mainly mediated by maternal uterus.