| 晚时相长时程增强翻转前后大鼠海马CA1区AMPAR/GluR2的表达 |
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| 引用本文： | 张莉,李延海,孟凯,谢雯.晚时相长时程增强翻转前后大鼠海马CA1区AMPAR/GluR2的表达[J].生理学报,2010(1). |
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| 作者姓名： | 张莉 李延海 孟凯 谢雯 |
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| 作者单位： | 西安交通大学医学院生理学与病理生理学系;西安交通大学环境与疾病相关基因教育部重点实验室;西安交通大学生命科学院生物医学工程系; |
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| 基金项目： | supported by the National Natural Science Foundation of China(No.30670680); the Medical Foundation for younger scholars of Xi'an Jiaotong University(No.YQN0811) |
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| 摘 要： | 晚时相长时程增强(late-phase long-term potentiation,L-LTP)对于海马长期记忆的维持具有非常重要的作用,然而L-LTP可被诱导之后的神经元活动所翻转。本实验旨在研究海马CA1区L-LTP的翻转是否有突触前机制的参与以及L-LTP翻转前后AMPARs的表达有无变化。实验采用海马脑薄片细胞外场电位记录技术,使用强直刺激(high-frequency stimulation,HFS)诱导出CA1区L-LTP,2h后用两组间隔10min的高强度的双脉冲低频刺激(high-intensity paired-pulse low frequency stimulation,HI-PP-LFS)诱导L-LTP翻转。在LTP诱导前、诱导2h后、翻转后均给予一个双脉冲刺激,观察双脉冲比值(paired-pulse ratio,PPR)的变化;另一方面,实验通过免疫荧光组织化学方法观察AMPAR/GluR2在L-LTP翻转前后海马CA1区表达的变化。结果显示,L-LTP诱导后2h,HI-PP-LFS可诱导L-LTP的部分翻转(翻转率为61.79%±14.51%)。LTP诱导前、诱导2h后、翻转后PPR均大于1,表现为双脉冲易化(paired-pulse facilitation,PPF),且三者大小顺序为:LTP诱导后LTP翻转后LTP诱导前;在海马CA1区AMPAR/GluR2亚单位的表达方面,对照组、LTP组及LTP翻转组之间没有显著差异。上述结果提示,海马CA1区L-LTP维持与翻转均有突触前机制的参与,但L-LTP诱导与翻转前后AMPAR/GluR2表达没有发生变化。
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| 关 键 词： | L-LTP LTP翻转 AMPARs/GluR2 海马 |
The expressions of AMPAR/GluR2 in hippocampal CA1 area of rats before and after late-phase long-term potentiation reversal |
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| ZHANG Li,LI Yan-Hai,MENG Kai,XIE Wen.The expressions of AMPAR/GluR2 in hippocampal CA1 area of rats before and after late-phase long-term potentiation reversal[J].Acta Physiologica Sinica,2010(1). |
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| Authors: | ZHANG Li LI Yan-Hai MENG Kai XIE Wen |
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| Institution: | ZHANG Li1,2,LI Yan-Hai3,MENG Kai1,XIE Wen1 1Department of Physiology , Pathophysiology,Medical School of Xi\'an Jiaotong University,2Ministry of Education Key Laboratory of Environment , Gene Related Diseases,Xi\'an Jiaotong University,Xi\'an 710061,China,3Institute of Biomedical Engineering,School of Life Science , Technology,Xi\'an 710049 |
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| Abstract: | Late-phase long-term potentiation (L-LTP) plays a very important role in the maintenance of long-term memory in hippocampus.However,studies have shown that L-LTP can be reversed by subsequent neuronal activity.The aim of the present study is to investigate whether the presynaptic mechanism and the change of AMPARs expressions are involved in the reversal of L-LTP in hippocampal CA1 area.Standard extracellular recording technique was used to record the potential change in the stratum radiatum of CA1 area of ... |
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| Keywords: | L-LTP LTP reversal AMPARs/GluR2 hippocampus |
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