Balb／c小鼠左右两侧大脑皮层IL—1β，IL—6含量的不对称性

为探讨左右侧大脑皮层白细胞介素1β（interdeukine-1β,IL-1β）和白细胞介素6（interleukine-6,IL-6)的含量状况及与左右侧大脑皮层免疫调控异质性的关系,。分别取正常及细胞菌脂多糖（lipopolysaccharuide,LPS)刺激2h后的Balb/c小鼠的左右侧大脑皮层,制备匀浆液,用ELISA法检测匀浆液中IL－1β,IL-6的含量,结果显示,正常小鼠大脑皮层IL－1β,IL-6含量均为右侧显著高于左侧;LPS刺激后,左侧大脑皮层IL－6含量明显升高,右侧大脑皮层IL－6含量显著高于左侧大脑皮层,而左右侧大脑皮层IL－1β含量无明显差异,该实验结果表明,Balb/c小鼠在正常生理状态下两大脑皮层IL－1β,IL－6含量存在不对称性,左右侧大脑皮层免疫调控异质性可能与细胞因子的不对称性有关。

Selective distribution of IL-1 beta and IL-6 contents in the right and left cortices of Balb/c mice

The purpose of the present work was to study the different distribution of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) contents in the right and left cortices, which may be related to the differences in immunomodulatory functions between the two cerebral cortices. Samples of tissue homogenate were prepared from the left and right cerebral cortices of the Balb/c mice and IL-1 beta, and IL-6 contents were measured using ELISA kits. Both IL-1 beta (P < 0.01) and IL-6 (P < 0.001) contents of the right cortex were higher than those of the left. After injection of LPS, the IL-6 content of the left cortex increased. No statistically significant difference of IL-1 beta contents was found between the two cerebral cortices, while the IL-6 content of the right cortex was higher than that of the left (P < 0.01), suggesting that some cytokines are probably involved in the differences between the right and left cerebral immunomodulatory functions in mice.