| 褪黑素抑制低氧引起大鼠大脑皮层氨基酸递质释放 |
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| 作者姓名： | Gu J Li XJ Sun FY |
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| 作者单位： | 1. 上海医科大学基础医学院神经生物学教研室、医学神经生物学国家重点实验室,上海,200032
2. 上海医科大学基础医学院神经生物学教研室、医学神经生物学国家重点实验室,上海,200032;上海生命科学研究中心,上海,200031 |
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| 摘 要： | 为研究褪黑素对低氧引起大鼠大脑皮层脑片氨基酸释放变化的影响，利用反相高效液相色谱结合荧光检测法，测定了孵育液中氨基酸类神经递质的含量。低氧条件为通入９１．６％Ｎ２和８．４％Ｏ２的混合气体。低氧３０ｍｉｎ时，大鼠大脑皮层脑片孵育中，氨基酸类神经递质天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸、牛磺酸和γ－氨基丁酸的含量显著增加，其含量分别是正常氧组的２４０．４％，３３４．３％，２００．６％，２１０．４％，１６
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| 关 键 词： | 褪黑素 低氧 脑片 兴奋性氨基酸 |
Melatonin decreases the release of amino acid transmitters in hypoxic rat cortical slice |
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| Gu J,Li XJ,Sun FY.Melatonin decreases the release of amino acid transmitters in hypoxic rat cortical slice[J].Acta Physiologica Sinica,1999,51(4):445-448. |
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| Authors: | Gu J Li X J Sun F Y |
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| Institution: | National Key Laboratory of Medical Neurobiology, Department of Neurobiology, Shanghai Medical University, Shanghai 200032. |
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| Abstract: | To study the effect of melatonin on changes in amino acid release following hypoxic stimulation, a RP-HPLC fluorometric method was used to measure the content of amino acids in the culture medium of rat brain slices. Hypoxic condition was instituted by a gas of 91.6% N2 and 8.4% O2. The results showed that the contents of amino acids, including aspartate, glutamate, glutamine, glycine, taurine and GABA, in the medium were significantly increased to 240.4%, 334.3%, 200.6%, 210.4%, 168.6% and 263.9%, respectively (P < 0.01 vs control) by 30 min hypoxic incubation. Melatonin can significantly reduce the increase of aspartate and glutamate, to 55.1% and 57.0% of the hypoxic group. |
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| Keywords: | melatonin hypoxia brain slice excitatory amino acids |
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