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| 基因"慢"的克隆及其在小鼠组织中的表达 | |
| 作者姓名： | Cui YZ Peng YF Tian Q |
| 作者单位： | 1. 俄勒冈医科大学环境与生命科学系,俄勒,冈州,97006,美国 2. 皖南医学院弋矶山医院生殖医学研究所,芜湖,241001 3. 美国俄勒冈医科大学病理学系,俄勒,冈州,97239,美国 |
| 基金项目： | This work was supported by the National Institutes of Health of USA.ACKN0WLEDGEMENTS： We thank Dr. Eric Davidson（California Institute of Technology） for generously allowing us to use the Q-bot colony arrayer and Dr, Mustafa Khokha for providing in situ probes and Dr. E. J. Robertson for BMP7 plasmid. This study was performed in University of Califomia, Berkeley. |
| 摘 要： | Formin蛋白家族由结构相关的蛋白组成，它们都有两个formin同源功能区（formin homology domains），FH1和FH2。这些基因上的多种变异均导致动物四肢畸形，提示这些基因在肢体发育中起重要作用。我们自小鼠肢体基因库中分离出了一个新基因：“慢”，该基因含有FH1和FH2。我们检测了该基因在小鼠胚胎及成体的表达情况，并对可能的功能意义进行了讨论。 |
| 关 键 词： | 基因克隆 小鼠 基因表达 肢体基因库 四肢畸形 胚胎发育 |
| 收稿时间： | 2005-03-30 |
| 修稿时间： | 2005-05-20 |
Expression of man, a formin homology domain-containing gene in the mouse limb |
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| Cui YZ,Peng YF,Tian Q.Expression of man, a formin homology domain-containing gene in the mouse limb[J].Acta Physiologica Sinica,2005,57(4):433-438. | |
| Authors: | Cui Yan-Zhen Peng Yi-Feng Tian Qi |
| Institution: | Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 NW Walker Rd., Beaverton, OR 97006, USA;Institute of Reproductive Medicine, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China;Department of Pathology, L113, School of Medicine, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA;E-mail: cuiy@ebs.ogi.edu. |
| Abstract: | Formin defines a family of structurally related proteins that have two formin homology domains (FH1 and FH2). Variousmutations in the formin locus result in limb deformity, suggesting that these genes play indispensable roles in the limb development.Here we report the isolation of a novel cDNA, man, from the mouse limb, which contains two conserved FH1 and FH2 domains. Itsexpression is described and possible functional significance is discussed. |
| Keywords: | man formin FH1 FH2 mouse limb cytokinesis expression cloning |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |