油酸对消炎痛引起的胃粘膜损伤大鼠胃粘液分泌的影响

本工作研究油酸对消炎痛引起胃粘膜损伤大鼠胃粘液分泌的影响。胃粘液测定采用阿尔新蓝(Alcian blue)与胃液中糖蛋白结合的方法。将1.0ml 油酸注入到结扎幽门的大鼠空肠内,就可引起胃壁粘液及游离粘液分泌量的明显增加。以0.25、0.5和1.0ml 油酸注入到不结扎幽门的大鼠空肠内,也能显著增加胃壁粘液分泌,保护胃粘膜。这两种作用表现着剂量依赖关系。不论以油酸灌胃或注入空肠、回肠,都能明显增加胃壁粘液量,而灌胃的作用比注入肠内更明显。以1.0ml 30%甘油、0.1%乙酸及1/15N HCl 分别注入空肠,都不能刺激胃壁粘液的分泌。上述结果表明,油酸具有刺激胃粘液分泌的作用。因此,加强胃粘液分泌可能对粘膜起到屏障作用,这是油酸保护胃粘膜损伤的机制之一。

INFLUENCE OF OLEIC ACID ON GASTRIC MUCUS SECRETION IN INDOMETHACIN-INDUCED GASTRIC MUCOSAL LESIONS IN RATS

The effect of oleic acid on gastric mucus secretion in rats with indomethacin-induced gastric mucosal lesions was studied.The mucus secretion was measured bycombination of Alcian blue with glucoprotein in gastric juice.The results were asfollows:(1)Oleic acid 1.0 ml introduced into the jejunum of Shay rats,increasedgastric barrier mucus(P<0.01)and gastric free mucus(P<0.05)significantly.(2)A significant increase of gastric barrier mucus and cytoprotection action wasobserved when 0.25,0.5 and 1.0 ml of oleic acid were introduced into the jejunum.Both these effects were dose-dependent.(3)Oleic acid introduced directly into thestomach was more effective in the increase of gastric barrier mucus than thatinstilled into the small intestine.(4)30% of glycerin,0.1% acetic acid or 1/15N HCl were without effect on the gastric barrier mucus secretion.These results clearly show that oleic acid stimulates secretion of gastric mucus.This effect may form a part of the mechanism involved in its cytoprotective action.