| 超氧化物歧化酶对内皮细胞缺氧复氧损伤的防护作用 |
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| 引用本文： | 董建文,时安云.超氧化物歧化酶对内皮细胞缺氧复氧损伤的防护作用[J].生理学报,1997,49(6):644-648. |
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| 作者姓名： | 董建文 时安云 |
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| 作者单位： | 北京医科大学病理生理教研室!北京，100083，北京医科大学病理生理教研室!北京，100083 |
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| 摘 要： | 体外培养扔兔胸主动脉内皮细胞缺氧３０ｍｉｎ后复氧１０ｍｉｎ，可以发现缺氧后复氧可引起细胞乳酸脱氢酶释放量，细胞悬液丙二醛含量增加，谷胱甘肽过氧化酶活性降低，细胞合成释放一氧化氮减少，细胞内钙离子浓度明显升高；ＥＣ的这些损伤在缺氧期间即有表现，复氧后更为加剧。而在缺氧前预先加入终浓度为２００Ｕ／ｍｌ的超氧化物歧化酶可改善细胞的抗氧化能力，减轻缺氧复氧对ＥＣ的损伤。
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| 关 键 词： | 内皮细胞 缺氧复氧 损伤 超氧化物歧化酶 |
PROTECTION OF SUPEROXIDE DISMUTASE ON HYPOXIA-REOXYGENATION INJURY TO ENDOTHELIAL CELL |
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| DONG JIAN-WEN, SHI AN-YUN.PROTECTION OF SUPEROXIDE DISMUTASE ON HYPOXIA-REOXYGENATION INJURY TO ENDOTHELIAL CELL[J].Acta Physiologica Sinica,1997,49(6):644-648. |
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| Authors: | DONG JIAN-WEN SHI AN-YUN |
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| Abstract: | Short term hypoxia induced endothelial cells (ECs) injury, as manifested in increasing lactate dehydrogenase (LDH) release and malondialdehyde (MDA) content,decreasing nitric oxide (NO) production and antioxidant enzyme glutathione peroxidase (GSH-Px) activity and increased intracellular calcium concentration, which were further exaggerated by reoxygenation. Administration of 200 U/ml superoxide dismutase (SOD) before hypoxia could partially prevent EC from such injuries, suggesting that the presence of oxygen free radicals may be one of the main factors involved in hypoxia-reoxygenation injury. The ameliorative effect of SOD in case is obviously due to elimination of oxygen free radicals. |
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| Keywords: | endothelial cell ischemia reperfusion injury hypoxia reoxgenation free radical nitric oxide superoxide dismutase |
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