内皮素-1对肺表面活性物质分泌的调控

采用离体大鼠非灌流肺,观察生理浓度内皮素－１对肺表面活性物质分泌的影响。结果表明,１０＾－１２和１０＾－１０ｍｏｌ／Ｌ的ＥＴ－１可促进ＰＳ磷脂及其主要组分磷脂酰胆碱的基础分泌;在间歇性肺扩张刺激基础上,ＥＴ－１可进一步加强磷脂和ＰＣ的分泌。蛋白激酶Ｃ抑制剂Ｈ７可阻断ＥＴ－１的促ＰＳ分泌效应。

Modulation of endothelin-1 on pulmonary surfactant secretion

The effects of endothelin-1 (ET-1 ) at physiological level on the secretion of pulmonmp surfactant (PS) were studied on isolated nonperfused rat lungs. The results showed that ET-1 (10-12 and 10-10 mol/L) increased the hasal secretion of total phospholipids (TP) and phosphatidylcholine (PC), and potentiated the stimulatory effect of intendttent lung inflation on the secretion of ac and PC. The effect of ET-l could be inhibited by H7, a protein kinase C (PKC) inhihitor. When the cultured lung tissue was exposed respectively to ET-1 (10-12 and 10-10 mol/L) for I h and 4 h,the content of ET in the lung tissue increased significantly. These results suggest that ET-l at the physiological concentration is involved in modulating PS secretion thlough a PKCdependent pathway, and can also promote ET production in lungs.