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成年鼠缺血性脑损伤诱导nestin的表达
作者姓名:Liu PC  Lu SD  Huang YL  Sun FY
作者单位:复旦大学上海医学院医学神经生物国家重点实验室,上海,200032
基金项目:This work was partly supported by grants from the National Nature Science Foundation of China (No. 39730170 and 398251
摘    要:应用免疫组化和免疫荧光双标技术结合激光共聚焦扫描显微镜,观察缺血性脑损伤后脑内nestin的表达及其细胞类型。实验观察结果为,再灌后1天,在缺血中心区可见nestin阳性突起;再灌后3天和1周时,除缺血中心区外,周边I、Ⅱ、Ⅲ区均有nestin阳性突起主要与GFAP共存;2周时,nestin阳性突起变粗、变长,并与NSE的共存明显增多。上述研究结果提示,脑缺血可诱导大鼠脑缺血区域表达nestin,该表达可能与神经细胞的修复有关。

关 键 词:成年鼠  缺血性脑损伤  诱导  nestin  表达  脑缺血  荧光双标  激光共聚焦扫描显微镜  巢蛋白
修稿时间:2001年12月18

The expression of nestin in ischemia-injured brain of adult rat
Liu PC,Lu SD,Huang YL,Sun FY.The expression of nestin in ischemia-injured brain of adult rat[J].Acta Physiologica Sinica,2002,54(4):294-299.
Authors:Liu Peng-Chong  Lu Shi-Duo  Huang Ya-Lin  Sun Feng-Yan
Institution:National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032.
Abstract:Immunohistochemistry and double immunofluorescent labeling techniques combined with confocal laser scanning microscope analysis were used to investigate the characteristic spatial induction profile of nestin following a transient middle cerebral artery occlusion in adult rat brain. The results showed that nestin was induced in ischemic core at 1 day after reperfusion. In addition to ischemic core, the expression of nestin increased in peri-ischemic I, II and III regions at 3 days and 1 week, then it decreased and narrowed along the rim of ischemic core 2 weeks after reperfusion. Double immunofluorescent labeling showed that nestin positive cells were mostly co-stained with GFAP,a astrocyte marker, in peri-ischemic I region 3 days after reperfusion. At 2 weeks, however nestin cells showed a long process and the cells double stained with nestin and NSE,a neuonal specific marker,increased in the ischemic brain. The results suggest that cerebral ischemia induces nestin expression in damaged neurons which might favor the neuroprotection against ischemic damage.
Keywords:cerebral ischemia  double immunofluorescent labeling  confocal laser scanning microscope  nestin
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