一氧化氮供体增强大鼠记忆及其与脑内ADP-核糖基转移酶的关系

为探讨与学习记忆有关的一氧化氮（ｎｉｔｒｉｃ ｏｘｉｄｅ,ＮＯ）信号转导通路,本文用ＮＯ供体硝普钠（ｓｏｄｉｕｍ ｎｉｔｒｏｐｒｕｓｓｉｄｅ,ＳＮＰ）或同时给予ＡＤＰ－核糖基转移酶（ＡＤＰ－ｒｉｂｏｓｙｌｔｒａｎｓｆｅｒａｓｅ,ＡＤＰＲＴ）抑制剂尼克酰胺（ｎｉｃｏｔｉｎａｍｉｄｅ,ＮＩＣ）侧脑室内流射,观察其对大鼠学习记忆行为的影响,并用高效液相色谱法测定脑内ＡＤＰＲＴ活性。结果表明,ＳＮＰ（０．

Relationship between memory enhancement induced by nitric oxide donor and brain ADP-ribosyltransferase in rats

In order to explore the signal transduction pathway of nitric oxide (NO) related to learning and memory, the effects of intracerebral ventricular injection (icv) of NO donor sodium nitroprusside (SNP) alone or along with ADP-ribosyltransferase (ADPRT) inhibitor nicotinamide (NIC) on learning and memory behavior were investigated in rats. Brain ADPRT activity was analyzed using high performance liquid chromatography. The results showed that SNP (0.36 microgram, icv) facilitated short-term and long-term memory in passive avoidance response and elevated learning and memory ability in active avoidance response. NIC (1.5 mg, icv) blocked the effect of SNP. Training in active avoidance task raised ADPRT activity in hippocampus. Treatment with SNP (icv) before training caused significant elevation of ADPRT activity in hippocampus and cerebral cortex. The results above support the view that ADPRT may be one of the target molecules of NO action.