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大鼠颈部淋巴引流阻断后海马bcl-2、bax表达和细胞凋亡的动态变化
作者姓名:Li YX  Xia ZL  Chen LB  Ye WJ  Yang MF  Sun QL
作者单位:1. 山东大学医学院生理研究所,济南,250012
2. 山东大学医学院生理研究所,济南,250012;泰山医学院脑微循环研究所,泰安,271000
3. 泰山医学院脑微循环研究所,泰安,271000
4. 泰山医学院生物技术制药教研室,泰安,271000
基金项目:This work was supported by the Natural Scientific Foundation of Shandong Province (No. Z2002C40)
摘    要:为了研究淋巴滞留性脑病中海马bcl-2、bax基因表达和细胞凋亡的动态变化,本实验用阻断大鼠颈部淋巴引流的方法制备淋巴滞留性脑病模型,术后1、2、3、5、7和14d处死动物,H&E染色观察海马组织结构变化,TUNEL荧光标记检测原位细胞凋亡,RT-PCR检测海马bcl-2和bax的mRNA表达。结果显示脑组织有水肿的结构变化,第5天最明显。海马TUNEL阳性细胞数从术后2d开始增多,5d达最高值。bax表达于术后1d开始增高,第2天即达最高值。bcl-2表达于术后1d开始降低,5d达最低值。第14天上述指标均恢复到对照组水平。研究表明,阻断颈部淋巴引流所导致的淋巴滞留性脑病中海马bcl-2和bax的表达发生变化,而且海马神经细胞的死亡以凋亡为主。

关 键 词:大鼠  Wistar  淋巴结切除  脑损伤  细胞凋亡  基因  bcl-2
修稿时间:2004年5月31日

The changes of bcl-2, bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics of rats
Li YX,Xia ZL,Chen LB,Ye WJ,Yang MF,Sun QL.The changes of bcl-2, bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics of rats[J].Acta Physiologica Sinica,2005,57(1):54-58.
Authors:Li Yu-Xian  Xia Zuo-Li  Chen Lian-Bi  Ye Wen-Jing  Yang Ming-Feng  Sun Qiao-Ling
Institution:LI Yu-Xian,XIA Zuo-Li,CHEN Lian-Bi,YE Wen-Jing,YANG Ming-Feng,SUN Qiao-Ling Institute of Physiology,Shandong University School of Medicine,Jinan 250012,China,Institute of Cerebral Microcirculation,Taishan Medical School,Taian 271000,China, Departement of Biotechnological Pharmaceutics,Taishan Medical School,Taian 271000,China
Abstract:To investigate the changes in bcl-2, bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics, the model of lymphostatic encephalopathy was established by occluding and removing both the superficial and deep cervical lymph nodes in rats. The animals were sacrificed at 1, 2, 3, 5, 7 and 14 d after operation. H and E staining was used to observe the structure of brain tissues and TUNEL staining was used to detect in situ cell apoptosis in the hippocampus. The expression of bcl-2 and bax in the hippocampus were examined by RT-PCR. The results showed that cerebroedema appeared at day 2 and was most serious at day 5 after the blockade of cervical lymphatics. The number of TUNEL positive cells began to increase at day 2 and reached the maximum at day 5. The expression of bax began to increase at day 1 and reached the maximum at day 2. The expression of bcl-2 began to decrease at day 1 and dropped to the minimum at day 5. The items mentioned above recovered to control level at day 14. These results suggest that lymphostatic encephalopathy following the blockade of cervical lymphatics result in changes in bcl-2 and bax expression in the hippocampus and that apoptosis is the main form of neuron death.
Keywords:rats  Wistar  lymph node excision  brain injuries  apoptosis  genes  bcl-2
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