| 抑制Beclin1促进H2O2诱导的神经胶质瘤U251细胞凋亡 |
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| 作者姓名： | Kong XX Zhang HY Chen ZQ Fan XF Gong YS |
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| 作者单位： | 温州医学院低氧医学研究所机能实验教学中心;温州医学院药学院; |
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| 基金项目： | the Science Startup Foundation of Wenzhou Medical School,China(No.QTJ09014); the Science and Technology Project of Education Department of Zhejiang Province,China(No.Y200906732) |
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| 摘 要： | 氧化应激能够引起细胞自噬和凋亡同时发生,但其中细胞自噬的作用仍不十分明确,研究表明Beclin 1作为调节前自噬体形成的关键基因,参与了胶质瘤氧化应激的损伤过程.为了探讨自噬在H2O2引起的神经胶质瘤U251细胞损伤中的作用,本文应用真核细胞转染技术将Psilencer3.1-siRNA-Beclin 1重组质粒转入人...
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| 关 键 词： | RNA干扰 Beclin1 自噬 凋亡 |
Inhibition of Beclin 1 enhances apoptosis by H2O2 in glioma U251 cells |
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| Kong XX,Zhang HY,Chen ZQ,Fan XF,Gong YS.Inhibition of Beclin 1 enhances apoptosis by H2O2 in glioma U251 cells[J].Acta Physiologica Sinica,2011,63(3):238-244. |
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| Authors: | Kong Xiao-Xia Zhang Hong-Yu Chen Zhao-Qin Fan Xiao-Fang Gong Yong-Sheng |
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| Institution: | KONG Xiao-Xia1,ZHANG Hong-Yu2,*,CHEN Zhao-Qin1,FAN Xiao-Fang1,GONG Yong-Sheng1 1Institute of Hypoxia Medical Research,Teaching Center of Functional Experiment,2Pharmacy School,Wenzhou Medical College,Wenzhou 325035,China |
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| Abstract: | Oxidative stress could induce apoptosis and autophagy process simultaneously,but the role of autophagy is still not clear.Beclin 1,a key gene regulating the preautophagosome formation,is involved in the injury induced by oxidative stress.To observe the role of autophagy in H2O2-induced injury of U251 cells,the recombinant plasmid Psilencer3.1-siRNA-Beclin 1 was transfected into U251 cells by eukaryotic cell transfection technique.Plasmid vector and cell culture medium were used as negative and control group... |
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| Keywords: | RNA interference Beclin 1 autophagy apoptosis |
| 本文献已被 CNKI PubMed 等数据库收录！ |
