| 大鼠骨髓间充质干细胞分化的内皮样细胞促进血管新生及Rho激酶的作用 |
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| 作者姓名： | Zheng HZ Zhao J |
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| 作者单位： | 广东医学院生理学研究室生理学教研室; |
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| 基金项目： | supported by the Natural Science Foundation of Guangdong Province,China(No.9152402301000028); Program of Science and Technology Commission of Zhanjiang Municipality,Guangdong Province,China(No.2006-3-3); Program of Science and Technology Commission of Dongguan Municipality,Guangdong Province,China(No.2008108101060) |
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| 摘 要： | 本文研究大鼠骨髓间充质干细胞分化生成的内皮样细胞(rat bone marrow mesenchymal stem cells-differentiated endothelial like cells,rBMSC-ECs)在血管新生中的作用及Rho激酶(Rho kinase,ROCK)活性抑制的影响。实验建立rBMSC-ECs与主动脉环体外共培养实验模型,设单纯血管环组、血管环与细胞共培养组和HA-1077低、中、高浓度组,HA-1077组在共培养的基础上分别在培养液中加不同浓度(10、30、60mmol/L)的ROCK特异抑制剂HA-1077。结果显示,培养第3天,血管环与细胞共培养组新生微血管数是单纯血管环组的1.3倍(P<0.05);HA-107710、30和60mmol/L组较共培养组分别减少57.70%、64.13%和48.23%(均P<0.01)。第6天,共培养组及HA-1077组rBMSC-ECs数量明显增加,并迁移至血管环周边,新生微血管生长缓慢;HA-1077组新生微血管数较共培养组明显减少。第9天,共培养组新生微血管部分增粗、增厚、延长,部分退化;一些rBMSC-ECs出芽,形成毛细血管...
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| 关 键 词： | Rho激酶 血管新生 间充质干细胞 主动脉环 内皮分化 骨髓 大鼠 |
Rat bone marrow mesenchymal stem cells-differentiated endothelial like cells facilitate angiogenesis and the Rho kinase contribution |
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| Zheng HZ,Zhao J.Rat bone marrow mesenchymal stem cells-differentiated endothelial like cells facilitate angiogenesis and the Rho kinase contribution[J].Acta Physiologica Sinica,2011,63(4):359-366. |
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| Authors: | Zheng Hui-Zhen Zhao Jing |
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| Institution: | ZHENG Hui-Zhen *,ZHAO Jing Department of Physiology,Guangdong Medical College,Dongguan 523808,China |
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| Abstract: | The aim of this study was to investigate the influence of endothelial like cells differentiated from rat bone marrow mesen- chymal stem cells (rBMSC-ECs) on angiogenesis and the effect of Rho kinase (ROCK) inhibitor using an in vitro model of cells co- cultured with rat aorta ring. Cell proliferation capability was detected by MTT method. The rBMSC-ECs were co-cultured with rat aorta ring in rat tail collagen and endothelial medium. A ROCK specific inhibitor, HA-1077 at different concentrations (0, 10, 30 a... |
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| Keywords: | Rho kinase angiogenesis mesenchymal stem cells aorta ring endothelial differentiation bone marrow rat |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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