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行为训练与小鼠海马突触蛋白磷酸化水平的相关性
引用本文:高静,王新光.行为训练与小鼠海马突触蛋白磷酸化水平的相关性[J].生理学报,1992,44(1):86-91.
作者姓名:高静  王新光
作者单位:南京大学生物系生理教研室,南京大学生物系生理教研室,南京大学生物系生理教研室,南京大学现代分析中心 南京 210008,南京 210008,南京 210008
摘    要:本文对梭箱回避行为训练是否能改变小鼠海马突触蛋白磷酸化水平进行了检定。在小鼠训练结束的不同时程制备突触体,以γ-~(32)P-ATP作为标记进行离体测定。结果如下:(1)正常小鼠各脑区突触蛋白磷酸化水平无显著差异;(2)学习训练刚结束时,海马突触蛋白磷酸化水平与学习训练成绩的优劣有相关性;(3)学习训练结束后24h,上述相关性更为明显,且成绩良好组右侧海马突触蛋白磷酸化水平显著高于左侧,而成绩低劣组相反。海马突触蛋白磷酸化的这种不对称现象的成因尚不清楚。这些结果表明,海马突触蛋白磷酸化反应可能是习得行为的分子机制。

关 键 词:海马  突触蛋白  行为训练  磷酸化

CORRELATION BETWEEN SYNAPTIC PROTEIN PHOSPHORYLATION IN HIPPOCAMPI AND SHUTILE BOX AVOIDANCE BEHAVIOR
GAO JING,WU FU-MEI,XIAO XIN-SHENG,WANG XIN-GUANG Biology.CORRELATION BETWEEN SYNAPTIC PROTEIN PHOSPHORYLATION IN HIPPOCAMPI AND SHUTILE BOX AVOIDANCE BEHAVIOR[J].Acta Physiologica Sinica,1992,44(1):86-91.
Authors:GAO JING  WU FU-MEI  XIAO XIN-SHENG  WANG XIN-GUANG Biology
Institution:Biology Department, Nanjing University.
Abstract:The present investigation was undertaken to detect whether protein phosphorylation levels of hippocampal synaptosomes in mice trained on shuttle box avoidance task would show some changes. The synaptosomes were prepared at different times after the conclusion of training and labelled with gamma-32P-ATP for in vitro determination. The results are as follows: (1) No significant differences were found in the phosphorylation of synaptosomal proteins in different brain areas in normal mice before training. (2) Immediately after the conclusion of shuttle box avoidance task there appears a correlation between protein phosphorylation levels in hippocampal synaptosomes and the degree of success of training. (3) 24 h after the completion of training the above relationship is more significant, and protein phosphorylation levels of right hippocampus in Good Score Group is markedly higher than that of left one, but in the Inferior Score Group, the left-right difference is reverse. The formation of such asymmetry in protein phosphorylation in hippocampi is unknown. These data indicate that the synaptic protein phosphorylation in hippocampi might be a molecular mechanism of acquisition.
Keywords:phosphorylation  hippocampus  synaptic proteins  shuttle box avoidance behavior  
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