在原代培养的大鼠肝细胞观察消炎痛的细胞保护作用

我们曾观察到消炎痛预处理能明显减轻四氯化碳和半乳糖胺对大鼠的肝损伤作用,本工作采用原代培养的大鼠肝细胞进一步观察了这一现象。结果表明,经消炎痛整体处理后分离的大鼠肝细胞,在原代培养的条件下,对四氯化碳的损伤仍然具有明显的抵抗作用,表现为细胞内酶的漏出少于对照组。正常大鼠离体肝细胞在原代培养条件下用消炎痛处理,在相当大的剂量和相当长的时间范围内未出现明显的抗损伤作用。结果提示:消炎痛整体处理可使肝细胞本身获得抗损伤的能力,而这种抗损伤能力的产生可能有赖于肝细胞外其它因素的参与。

OBSERVING CYTOPROTECTION OF INDOMETHACIN ON PRIMARY CULTURED HEPATOCYTES OF RATS

We observed previously that indomethacin induced hepatic protection againstCCl_4,and galactosamine in rats.In this work we further investigated this pheno-menon on primary cultured rat hepatocytes.The results showed that culturedhepatocytes of rats pretreated with indomethacin in vivo still retained the abilityresisting CCl_4-induced cytotoxicity,as indicated by lower leakage of enzymes.How-ever,cultured hepatocytes of normal rats treated with indomethacin in vitro had noobvious resistance to CCl_4,-induced cytotoxicity The results suggest that indome-thacin pretreatment in vivo enables hepatocytes to resist injury,which probablyalso needs participation of other factors.