Extracellular Oxidases of the Lignin-Degrading Fungus <Emphasis Type="Italic">Panus tigrinus</Emphasis> |
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Authors: | Email author" target="_blank">D?A?CadimalievEmail author V?V?Revin N?A?Atykyan V?D?Samuilov |
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Institution: | (1) Department of Biotechnology, Biological Faculty, Ogarev Mordovian State University, Bolshevistskaya ul. 68, 430000 Saransk, Russia;(2) Department of Physiology of Microorganisms, Biological Faculty, Lomonosov Moscow State University, 119899 Moscow, Russia |
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Abstract: | Two extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaldazine. The enzymes have identical pH (7.0) and temperature (60–65°C) optimums. Absorption spectra of the oxidases differ from the spectra of typical “blue” laccases and are similar to the spectrum of yellow oxidase.__________Translated from Biokhimiya, Vol. 70, No. 6, 2005, pp. 850–854.Original Russian Text Copyright © 2005 by Cadimaliev, Revin, Atykyan, Samuilov. |
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Keywords: | lignin biodegradation oxidase laccase white rot fungi Panus (Lentinus) tigrinus |
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