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Kinetic measurement of bacterial adherence to eukaryotic cells |
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| Authors: | AL Smith PD Jenny L Langley P Verdugo M Villalon J Unadkat D Luchtel |
| Institution: | a Division of Infectious Disease, Children's Hospital and Medical Center; Department of Pediatrics, University of Washington, Seattle, Washington, USA b Biological Structure; Center for Bioengineering, University of Washington, Seattle, Washington, USA c School of Pharmacy; University of Washington, Seattle, Washington, USA d School of Public Health, University of Washington, Seattle, Washington, USA |
| Abstract: | We developed a kinetic assay using a monolayer of differentiated respiratory epithelium in culture to assess bacterial adherence. Mean residence time of bacteria in the tissue culture chamber was estimated from a model-independent (moment) analysis of the rate of bacterial washout from perfused Rose chambers. Results with this method compared favorably with visual assessment of adherence and double radiolabel method with H. influenzae. Adherence was assessed with low inoculae of H. influenzae, P. cepacia and P. aeruginosa avoiding cytotoxic effects seen when large inoculae are added to eukaryotic cells. This method will provide a means of assessing adherence of pathogenic respiratory bacteria to their cellular target at low inoculae. |
| Keywords: | Bacterial adherence Respiratory epithelium |
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