Immunological biochips for studies of human erythrocytes |
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| Authors: | A V Shishkin I I Shmyrev S A Kuznetsova N G Ovchinina A A Butylin F I Ataullakhanov A I Vorob’ev |
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| Institution: | (1) National Research Center for Hematology, Russian Academy of Medical Sciences, Novozykovskii proezd 4a, Moscow, 125167, Russia;(2) Izhevsk State Medical Academy, ul. Kommunarov 281, Izhevsk, 426034, Russia;(3) Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russia;(4) Center for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia |
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| Abstract: | Immunological microarrays (biochips) for detecting erythrocyte surface antigens, viz., blood group antigens (A, B, 0) and Rhesus system antigens (D, E, e, C, and c), are described. The biochips represent transparent plastic supports onto which 1.5-mm spots of specific immobilized antibodies (IgM) are coated in different dilutions. The volume of tested blood samples is rather small (1–2 μl). Binding of erythrocytes to antibodies immobilized on the biochips is specific and allows further morphological analysis of bound cells. Analysis of the dynamics of cell detachment from biochip spots using a microfluidic chamber at different flow rates of the washing solution showed that combination of a biochip with a microfluidic chamber is a promising approach to concentration of cells of various immunotypes even if their content in the mixture is very low. |
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