B chromosomes of Korean field mouse Apodemus peninsulae (Rodentia,Murinae) analysed by microdissection and FISH |
| |
Authors: | Karamysheva T V Andreenkova O V Bochkaerev M N Borissov Y M Bogdanchikova N Borodin P M Rubtsov N B |
| |
Institution: | Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia. |
| |
Abstract: | Organization of B chromosomes in the Korean field mouse Apodemus peninsulae was analyzed. We painted its metaphase chromosomes with whole and partial chromosome paints generated by microdissection and DOP-PCR. The results of the painting indicated that all B chromosomes contained a large amount of repeated DNA sequences. The repeats could be classified in terms of their homology and predominant location. Pericentromeric repeats of B chromosomes were present in many copies in pericentromeric C-blocks of all autosomes and in non-centromeric C-blocks of the sex chromosomes. B arm specific type 1 repeats comprised the main body of the arms of almost all B chromosomes and were present in the arms of A chromosomes as interspersed sequences. B arm-specific type 2 repeats were found at the ends of some B chromosomes that did not undergo compaction at the interphase- metaphase transition and remained uncondensed. On the basis of comparative analysis of localization of B chromosome repeats in the chromosomes of two related species, A. peninsulae and A. agrarius, we suggest a hypothesis of B chromosome origin and evolution in the genus Apodemus. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|