首页 | 本学科首页   官方微博 | 高级检索  
   检索      

狗牙根颖果胚性愈伤组织的诱导和胚性细胞的超微结构及植株再生
引用本文:谢海燕,毛碧增,单兰兰,陈毓荃.狗牙根颖果胚性愈伤组织的诱导和胚性细胞的超微结构及植株再生[J].植物生理与分子生物学学报,2004,30(2):209-215.
作者姓名:谢海燕  毛碧增  单兰兰  陈毓荃
作者单位:1. 浙江大学生物技术研究所,杭州,310029;西北农林科技大学生命科学学院,杨凌,712100
2. 浙江大学生物技术研究所,杭州,310029
3. 西北农林科技大学生命科学学院,杨凌,712100
基金项目:杭州禾大生物技术工程有限公司资助项目
摘    要:以普通狗牙根Cynodon dacylon(L.)Pers.cv.'Suncitv']颖果为外植体,以MS为基本培养基,外加浓度在2.0~6.0mg/L的2,4-D,能高频率地诱导出高质量的胚性愈伤组织,其中以4.0 mg/L为最佳.胚性愈伤组织最佳继代及分化的培养方法为:用MS 2,4-D 4.0mg/L继代1~2次,然后转入1/2 MS 2,4-D 2.0 mg/L中继代1~2次,再在无激素的1/2MS中光照培养10 d,最后在MS 6-BA 3.0 mg/L中诱导分化,分化成苗率达31.7%.经电镜观察发现,胚性愈伤组织结构紧密,细胞较小,内容物丰富,而非胚性愈伤组织结构疏松,细胞巨大,内含一大液泡,几无细胞器.

关 键 词:普通狗牙根  颖果  胚性愈伤组织  再生植株  超微结构
修稿时间:2003年10月14

Induction of Embryogenic Calli from Caryopses of Common Bermudagrass and Ultrastructure of Embryogenic Cells as well as Plant Regeneration
XIE Hai-Yan,MAO Bi-Zeng,SHAN Lan-Lan,CHEN Yu-Quan.Induction of Embryogenic Calli from Caryopses of Common Bermudagrass and Ultrastructure of Embryogenic Cells as well as Plant Regeneration[J].Journal Of Plant Physiology and Molecular Biology,2004,30(2):209-215.
Authors:XIE Hai-Yan  MAO Bi-Zeng  SHAN Lan-Lan  CHEN Yu-Quan
Institution:Biotechnology Institute, Zhejiang University, Hangzhou 310029, China.
Abstract:A tissue culture system for embryo-genie callus (EC) induction and plant regeneration of commonbermudagrassusingmaturecaryopsis (embryos) as explants was developed. The results showed that embryogenic calli could be induced from caryopsis with high frequency,in MS medium with 2,4-D 2.0-6.0 mg/L (Table 1), and the best concentration of 2,4-D was 4.0 mg/L. The best method for maintaining EC and tissue differentiation was to subculture EC in MS 2, 4-D 4.0 mg/L 1-2 times, follwed by subcultur-ing in 1/2 MS 2,4-D 2.0 mg/L for 1-2 times, then to transfer EC to 1/2 MS without hormone for a 10-d-preregeneration in light, followed by transferring to MS 6-BA 3.0 mg/L for regeneration, with regeneration frequency 31.7% (Table 2). Morphological and micro-structural differences between EC and non-embryogenic callus (NEC) were observed by electron microscope (Fig.2). Ultrastructrual characteristics of the EC cells are described in this paper.
Keywords:common bermudagrass  caryopsis  embryogenic callus  regenerated plantlet  ultrastructrue
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号