T-DNA插入产生的水稻迟抽穗突变体的遗传分析

在筛选和鉴定水稻T-DNA插入纯合体的过程中,观察到一个迟抽穗的突变体.对该突变体进行遗传分析的结果表明,分离群体后代中出现正常抽穗、迟抽穗和特迟抽穗3种类型,分离比率为1:2:1,符合一对基因的不完全显性遗传;对突变体及其后代分离群体做Basta抗性检测及PCR分子检测,证实该突变体是由T-DNA插入所引起的,突变性状与T-DNA共分离.该材料可用于插入座位的基因克隆.

Genetic Analysis of a Heading-delayed Mutant by T-DNA Insertion in Rice (Oryza sativa L.)

Transposon tagging was used to iso-late genes in higher plant. In this study, a de-layed heading mutant caused by T-DNA inser-tion in rice was identified. Genetic analysis ofthe mutant showed that the three types ofphenotype, normal heading, delayed heading andoverly delayed heading (Table 1, Figs.1 and 2)in the segregating populations derived from theT-DNA heterozygotes fit the ratio of 1:2:1(Table2). Test for Basta resistance showed the delayedheading plants were all resistant while the nor-mal heading plants were susceptible, and the ra-tio of resistant and susceptible plants was 3:1(Table 3), which indicated that the delayed head-ing mutant was co-segregated with Basta resis-tance (Table 4). The delayed heading mutantcaused by T-DNA insertion was confirmed by T-DNA detection using PCR method (Fig.3, Table5). This delayed heading mutant will be used forisolation of the tagged gene in rice.