水稻Ds插入纯合体的筛选和鉴定

采用Basta抗性鉴定、潮霉素抗性鉴定和PCR检测相结合的方法筛选和鉴定了水稻Ds插入纯合体。在T1代236个转化株系中，有16个株系的全部植株表现出对Basta的敏感，其余220个株系的植株表现出对Basta的抗性。经过3代的纯合筛选，共鉴定出Ds插入纯合体203个．这些Ds插入纯合体可用于构建Ac／Ds系统和对Ds插入突变体进行筛选和鉴定，为水稻功能基因组学研究提供了材料。

Selection and Identification of Ds Insertion Homozygotes in Rice (Oryza sativa L.)

Transposon tagging is a useful approach for gene isolation in higher plant.Its first step is the development of Ac/Ds system. In this paper, the identification of the homozygotes with Ds insertion in rice ( Oryza sativa L.) by a method combining Basta resistance, hygromycin resistance and PCR polymorphism (Fig.1) is described. A total of 236 transformed lines were subjected to Ds homozygote selection.In T 1 generation, 220 lines were found to be resistant to Basta and 16 lines were susceptible (Table 1). After two generations of selfing of the 220 Basta-resistant lines, 203 Ds homozygotes were identified and the other 17 lines were still segregating (Tables 2, 3). The Ds homozygotes can be used to develop Ac/Ds system and to detect mutants for rice functional genomic study.