花生种子发育和萌发过程中贮藏蛋白的合成和降解

以花生品种汕油5 2 3种子为材料,分离纯化花生球蛋白的41 kD和38.5 kD两种主要亚基及伴花生球蛋白的6 0.5 KD亚基并制备抗体.We stern blot分析表明,3种亚基在花生胚组织分化期的胚轴和子叶中就开始合成,其中60.5 kD亚基是最先在胚轴和子叶中大量合成和积累的贮藏蛋白,41 kD和38.5 kD亚基在随后的发育中积累量不断增加;种子萌发时这3种亚基的降解进程不一样,胚轴和子叶中41 kD和38.5kD亚基的降解均先于60.5 kD亚基.

Synthesis and Degradation of the Peanut Storage Proteins During Seed Development and Germination

Three polypeptides, 41 kD and 38.5kD subunits of arachin and 60.5 kD subunit ofconarachin in peanut (Arachis hypogaea L.Shanyou 523) seeds were purified by gel filtra-tion and SDS-PAGE (Fig.1). Polyclonal antibod-ies against these subunits were raised in mice( Table 1). Western blot showed that the subunitsappeared in axes and cotyledons at the tissuedifferentiation stage. The 60.5 kD subunit wasfirstly synthesized and accumulated in consid-erable quantity in axes and cotyledons, and thenthe 41 kD and 38.5 kD subunits increased dur-ing the development of peanut embryos (Fig.2).The degradation patterns of these three subunitswere different during the germination of peanutseeds. The 41 kD and 38.5 kD subunits in theaxes and cotyledons were degraded earlier thanthe 60.5 kD subunit (Fig.3).