| 拟南芥复制因子C亚基1在胚胎发生中起重要作用 |
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| 引用本文： | 夏石头,萧浪涛,毕冬玲,朱兆海.拟南芥复制因子C亚基1在胚胎发生中起重要作用[J].植物生理与分子生物学学报,2007,33(3):179-187. |
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| 作者姓名： | 夏石头 萧浪涛 毕冬玲 朱兆海 |
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| 作者单位： | 湖南农业大学湖南省植物激素与生长发育重点实验室 长沙410128(夏石头，萧浪涛，朱兆海)，北京生命科学研究所 北京102206(毕冬玲) |
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| 摘 要： | 复制因子C包含1个大亚基和4个小亚基,在DNA复制、损伤修复和细胞增殖中起重要作用,拟南芥复制因子C亚基1(AtRFC1)是人类复制因子C大亚基p140的同源蛋白。在对3个复制因子C亚基1的T-DNA插入突变株系rfc1-1、rfc1-2和rfc1-3的检验中,证实插入位点分别位于第16、19号外显子和启动子区域。T-DNA在外显子中的插入突变引起胚胎发育异常并导致胚胎和种子败育。将野生型拟南芥复制因子C亚基1基因转化到突变株系rfc1-1和rfc1-2后恢复了突变株的野生型表型,证明胚胎发生异常表型是由拟南芥复制因子C亚基1基因突变所引起的,AtRFC1在拟南芥胚胎发生中起重要作用。
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| 关 键 词： | 拟南芥复制因子C亚基1基因(AtRFC1) 胚胎发生 拟南芥 |
| 修稿时间： | 2006-11-062007-04-27 |
Arabidopsis Replication Factor C Subunit 1 Plays an Important Role in Embryogenesis |
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| XIA Shi-Tou,XIAO Lang-Tao,BI Dong-Lin,ZHU Zhao-Hai.Arabidopsis Replication Factor C Subunit 1 Plays an Important Role in Embryogenesis[J].Journal Of Plant Physiology and Molecular Biology,2007,33(3):179-187. |
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| Authors: | XIA Shi-Tou XIAO Lang-Tao BI Dong-Lin ZHU Zhao-Hai |
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| Institution: | 1.Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China; 2National Institute of Biological Sciences, Beijing 102206, China |
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| Abstract: | Replication factor C(RFC),consisting of one large subunit and four small subunits,is an important fac- tor involved in DNA replication and repair mechanisms as well as cell proliferation.The subunit 1 of Arabidopsis RFC(AtRFC1)is a homologue of p140,the large sub- unit of human RFC.Three T-DNA insertion mutant lines of AtRFC1,i.e.rfc1-1,rfc1-2 and rfc1-3,with insertion mutations located in exons 16 and 19,and the promoter region respectively were verified.These mutations caused defects in embryogenesis and led to embryo and seed abortion.Transformation of wild type AtRFC1 gene into rfc1 mutant alleles reverted the mutant phenotypes,sug- gesting that AtRFC1 plays an important role in embryo development in Arabidopsis thaliana. |
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| Keywords: | p140 AtRFC1 p140 embryogenesis Arabidopsis thaliana |
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