用分子标记技术分析不同生态型芦苇的遗传多样性

用分子标记技术对河西走廊4种不同生态型芦苇进行了遗传多态性分子标记分析。从30条ISSR引物和45条RAPD引物中分别筛选出适合4种不同生态型芦苇分析的9条和13条引物,前者共扩增出99条带,多态性位点数为51;后者共扩增出195条带,多态性位点数为87。两种分子标记分析所得的遗传相似系数呈显著正相关(r=0.845,P<0.05)。4种生态型芦苇表现出由水生芦苇经盐渍芦苇向沙丘芦苇逐渐演化的趋势。

An Analysis of Genetic Diversity of Different Ecotypes of Reed(Phragmites communis Trin.)by Molecular Marker Techniques

ISSR (inter-simple sequence repeat) and RAPD (random-amplified polymorphic DNA) markers were used to detect genetic diversity of 4 different ecotypes of reed (Phragmites communis Trin.) growing in Hexi Corridor,Gansu province (Table 1). Nine effective primers were screened from 30 ISSR arbitrary primers,and a total of 99 DNA bands were amplified,among which 51 (51.5%) were polymorphic (Table 3 and Fig.1). Thirteen effective primers were screened from 45 RAPD 10-oligonucleotide arbitrary primers,and a total of 195 DNA bands were amplified,among which 87 (44.6%) were polymorphic (Table 4 and Fig.1). Genetic identity based on ISSR and RAPD data showed a positive correlation (r=0.845,P<0.05) (Tables 5,6). Based on unweighted pair-group method with arithmetic averages (UPGMA) clus-ter analysis on DNA bands amplified (Fig.2),to-gether with the correlation analysis between ge-netic distance and soil water contents and soluble salt contents (Table 2) as well,the present results suggest that the genetic diversity occurs among the four ecotypes of reed in adaptation to long term natural drought and salinity,showing an obvious evolutional tendency from swamp reed via salt meadow reed to dune reed.