| 外源GUS基因在PEG介导的花椰菜原生质体中的瞬间表达 |
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| 引用本文： | 杨仲南,许智宏,白永延,卫志明.外源GUS基因在PEG介导的花椰菜原生质体中的瞬间表达[J].植物生理与分子生物学学报,1994(3). |
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| 作者姓名： | 杨仲南 许智宏 白永延 卫志明 |
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| 作者单位： | 中国科学院上海植物生理研究所 |
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| 摘 要： | 为了将外源基因导入花椰菜原生质体获得转基因植株，本文研究了ＰＥＧ介导的外源基因在花椰菜下胚轴原生质体中的瞬间表达。（１）２０％ＰＥＧ将质粒ｐＢＩ２２１导入原生质体后ＧＵＳ表达比１３％ＰＥＧ导入的高，但易造成原生质体损伤。（２）热激处理增强表达，但在随后的培养过程中易造成原生质体降解。（３）原生质体状况对表达有重要影响，５ｄ龄下胚轴原生质体比８ｄ龄的表达强。（４）不同质粒及启动子表达强度不同。质粒ｐＫＩＷＩ１０１比ｐＢＩ２２１表达强３倍左右。
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| 关 键 词： | 花椰菜，下胚轴，原生质体，PEG，瞬间表达 |
The Transient Gene Expression of Exogenous GUS Genes in Cauliflower Hypocotyl Protoplasts |
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| YANG Zhong-Nan, XU Zhi-Hong, BAI Yong-Yan and WEI Zhi-Ming.The Transient Gene Expression of Exogenous GUS Genes in Cauliflower Hypocotyl Protoplasts[J].Journal Of Plant Physiology and Molecular Biology,1994(3). |
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| Authors: | YANG Zhong-Nan XU Zhi-Hong BAI Yong-Yan and WEI Zhi-Ming |
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| Abstract: | In order to obtain transgenic plants from tranSformed cauliflower hypocotyl protoplasts, several factors affecting PEG mediated DNA transfer and protoplast survival were studied.(1) The transient expression of GUS gene mediated by 20% PEG was higher than that mediated by 13% PEG.However,13% PEG was more benificial than 20% PEG for high prtotoplast survival;(2) Heat shock improved the expression,but it was harmful to protoplasts;(3) The state of protoplasts affected the expression greatly.The Gus gene expression in protoplasts isolated from 5-day-old hypocotyls was much stronger than that from 8-dey old hypocotyls;(4) Plasmid pKI Wl101 gave a much stronger transient expression than plasmid PBI221. |
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| Keywords: | cauliflower hypocotyl protoplast PEG transient expression |
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