利用RNA干扰技术获得晚抽薹开花转基因大白菜

LEAFY基因在植物的成花转变中起重要作用。在拟南芥中,降低LEAFY基因的表达使开花延迟,并且增加分枝。利用RNAi技术降低大白菜LEAFY基因表达,获得晚抽薹开花的转基因大白菜。开花时转基因大白菜的株高明显低于对照,而叶片数、叶面积和侧枝数则高于对照;转基因植株的LEAFY基因表达明显降低,导致大白菜抽薹开花延迟。因此可以通过降低LEAFY基因表达来延迟需低温春化作用长日植物开花。也为利用生物技术获得晚抽薹转基因大白菜提供了理论依据。

Late-Bolting Transgenic Chinese Cabbage Obtained by RNA Interference Technique

LEAFY(LFY)gene plays an important role in determining plant flowering mainly by controlling the timing of phase transition.Constitutive under-expression of LFY in Arabidopsis resulted in the formation of a late- flowering and highly branching phenotype.In this paper, an RNAi approach was used in down-regulated LFY gene expression to delay Chinese cabbage(Brassica rapa L. ssp.pekinensis)bolting and flowering.The results show that transgenic plant has a later transition to the repro- ductive phase,and the transgenic plants have more branches,more leaves,but a lower height.Results of RQ- RT-PCR analysis show that LFY gene expression was greatly reduced in transgenic plants.These results sug- gest that inhibiting LFY gene expression by RNA inter- ference can delay bolting in a cold-sensitive long-day (LD)condition.Late flowering of Chinese cabbage can be used as a good genetic resource for the breeding late- bolting Chinese cabbage