| 手掌参内质网膜转运通道蛋白基因GcSec61β的克隆与表达 |
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| 引用本文： | 杨足君,周建平,李光蓉,张勇,任正隆.手掌参内质网膜转运通道蛋白基因GcSec61β的克隆与表达[J].植物生理与分子生物学学报,2007,33(4):354-360. |
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| 作者姓名： | 杨足君 周建平 李光蓉 张勇 任正隆 |
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| 作者单位： | 电子科技大学生命科学与技术学院 成都610054(杨足君，周建平，李光蓉，张勇)，电子科技大学生命科学与技术学院 成都610054四川农业大学植物遗传育种重点实验室雅安625014(任正隆) |
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| 基金项目： | 国家自然科学基金项目(No.30671288)，教育部春晖计划项目(No.2004251008)，教育部新世纪优秀人才计划(No.NCET-06-0810)，留学回国人员科研启动基金(No.LKQ0603)资助~~ |
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| 摘 要： | 从高原耐寒珍稀植物手掌参(Gymnadenia conopsea)中克隆了一个编码107个氨基酸、长为621bp的全长cDNA序列。序列分析表明,它与真核生物内质网(ER)转运蛋白通道亚基Sec61β基因具有高度的相似性,命名为GcSec61β。进化分析的结果表明GcSec61β与拟南芥和水稻等Sec61β基因的遗传关系最近。经半定量RT-PCR检测得知,GcSec61β基因在手掌参幼芽和叶中均有表达,其表达量受低温诱导。GcSec61β基因的原核表达具有明显增加细菌耐低温特性。
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| 关 键 词： | 手掌参 Sec61β基因 半定量RT-PCR 基因表达 |
| 修稿时间： | 2006-12-30 |
Cloning and expression of Gymnadenia conopsea GcSec61beta gene encoding endosplasmic reticulum membrane translocation channel protein] |
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| YANG Zu-Jun, ZHOU Jian-Ping, LI Guang-Rong, ZHANG Yong, REN Zheng-Long.Cloning and expression of Gymnadenia conopsea GcSec61beta gene encoding endosplasmic reticulum membrane translocation channel protein][J].Journal Of Plant Physiology and Molecular Biology,2007,33(4):354-360. |
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| Authors: | YANG Zu-Jun ZHOU Jian-Ping LI Guang-Rong ZHANG Yong REN Zheng-Long |
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| Institution: | School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn |
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| Abstract: | Protein translocation channel in endosplasmic reticulum (ER) of eukaryotes is composed of several subunits of Sec61 complex, which is essential for protein secretion. In the present study, we cloned a full-length cDNA fragment of 621 bp coding 107 amino acids from a psychrophile and endangered plant Gymnadenia conopsea, which grows in high land. Sequence analysis revealed that the gene was highly homologous to the member Sec61beta of ER protein transporter channel, which was thus designated as GcSec61beta. Phylogenetic tree shows that the GcSec61beta was closely related to the corresponding genes from Arabidopsis thaliana and Oryza sativa. Results of semi-quantitative RT-PCR showed that the expression of GcSec61beta was high both in leaves and the bud, and also induced by low temperature treatment. The sequence of the GcSec61beta was introduced into pET28a vector and transformed to E. coli strain BL21. The growth of E. coli was slowed down but the cold resistance was increased by the expression of GcSec61beta, which provides a new function of GcSec61beta protein. |
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