钙调素对花粉萌发和花粉管生长的效应

牛脑和玉米胚ＣａＭ能显著促进花粉萌发和花粉管生长（图１），而ＣａＭ抑制剂ＴＦＰ、ＣＰＺ及另外两个专一性更强的抑制剂Ｃｏｍｐｏｕｎｄ４８／８０和Ｗ７均严重抑制甚至阻止花粉的萌发（图２，３）。用对ＣａＭ亲和性较低的Ｗ７同系物Ｗ５，在与Ｗ７同样浓度下，对花粉萌发和花粉管生长无明显影响。此外，Ｗ７对花粉萌发和花粉管生长的抑制效应可被外源ＣａＭ所消除（图４）。在花粉萌发过程中，其内源ＣａＭ含量显著上升，在花粉萌发率接近最大值时，花粉ＣａＭ含量达最高水平（图５）。上述结果表明ＣａＭ对花粉萌发和花粉管生长的调控起重要作用。

Involvement of Calmodulin in Pollen Germination and Pollen Tube Growth

Calmodulin from both bovine brain and maize germs significantly promoted pollen germination and tube growth of Pinus yunnanensis and Nicotiana tabacum;CaM antagonists TFP and CPZ,and two other more specific drugs,W7 and compound 48/80,inhibited or stopped pollen germination and tube growth ;while W5,a less active analogue of W7,had little effect.The inhibitory effects of W7 could be abolished partiallyor totally by exogenous CaM.In addition,the CaM content in pine pollen increased significantly during pollen germination and reached a maximum when the percentage of germinated pollen was near its maximum.The results indicate that CaM plays an important role in the regulation and control of pollen germination and pollen tube growth.