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| 用mRNA差异显示法分离冷胁迫香蕉幼苗叶片内水杨酸诱导表达的基因 | |
| 引用本文： | 康国章,朱国辉,彭新湘,孙谷畴,王正询.用mRNA差异显示法分离冷胁迫香蕉幼苗叶片内水杨酸诱导表达的基因[J].植物生理与分子生物学学报,2004,30(2):225-228. |
| 作者姓名： | 康国章 朱国辉 彭新湘 孙谷畴 王正询 |
| 作者单位： | 1. 河南农业大学国家小麦工程技术研究中心,郑州,450002;中国科学院华南植物研究所,广州,510650 2. 华南农业大学生命科学学院,广州,510642 3. 中国科学院华南植物研究所,广州,510650 4. 广州大学生物系,广州,510405 |
| 基金项目： | Supported by a key research grant(No.1 999-11)from the Educational Committee of Guangzhou City,China |
| 摘 要： | 用mRNA差异显示法对7℃低温胁迫3 d后的香蕉幼苗叶片进行研究,回收了18条在低温下水杨酸(salicylic acid,SA)诱导的差异带;反向Northern杂交证明,SA在低温胁迫下能诱导7条差异带高表达.对其中最显著表达的两条差异带(G和A)进行克隆和测序,结果显示,G片段序列与大豆在冷胁迫环境下两个高表达基因片段的部分序列有92%同源性;A片段未发现其同源性基因片段. |
| 关 键 词： | 香蕉 抗寒性 mRNA差异显示 基因表达 水杨酸 |
Isolations of Salicylic Acid-induction-expressed Genes in Chilling-stressed Banana Seedling Leaves Using mRNA Differential Display |
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| Abstract.Isolations of Salicylic Acid-induction-expressed Genes in Chilling-stressed Banana Seedling Leaves Using mRNA Differential Display[J].Journal Of Plant Physiology and Molecular Biology,2004,30(2):225-228. | |
| Authors: | Abstract |
| Institution: | National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, China. |
| Abstract: | The mRNA differential display method was applied to identify banana genes that were regulated by salicylic acid (SA) during chilling stress. Eighteen cDNA fragments induced by SA during chilling stress were retracted. Seven of them were affirmed by reverse Northern hybridization to be significantly induced. Two most differential fragments (G and A) of them were cloned and sequenced. Nucleotide sequence analysis showed that clone G fragment had 92% homology to partial cDNA sequences of two cold-related genes in soybean (Glycine max) and clone A did not show any identity to previously reported sequences in GenBank database. |
| Keywords: | banana chilling tolerance mRNA differential display gene expression salicylic acid |
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