红豆草抗羟脯氨酸变异系原生质体培养后愈伤组织的抗性特征

通过NaN3诱变得到的红豆草抗羟脯氨酸（Hyp）变异系，在酶液中游离原生质体进行培养，获得再生植株。在含不同浓度NaCl、羟脯氨酸或PEG的MS培养基上，原生质体来源抗性意伤组织中的游离脯氨酸含量在1周之内均急剧增加，随后开始下降，3周后接近正常水平。随着胁迫程度的提高，抗性愈伤组织中游离脯氨酸含量呈递增趋势，生长速度呈递减趋势。

Resistant Characteristics of the Hydroxyproline Resistant Cell Line in Onobrychis viciaefolia After Protoplast Culture

The hydroxyproline resistant cellline of Onobrychis viciaefolia had been selected through NaN3 mutagenesis. Pramtoplast-derived plantlets of the resistantcell line were regenerated in fourmonths and were found to regain stronghydroxyproline resistance. After thecalli of the resistant cell line derivedfrom protoplasts were treated with different concentrations of NaCl, Hyp orPEG, the free proline content increasedsteeply in the first week and descendedto normal level in three weeks. In contrast to this, proline accumulation inthe wild tissue was not found at low selection level it could stand. Along withthe enhancement of stress, the free pramline concentration in calli of the resistant cell line increased, while the relative growth rate descended (Figs. 1 -6 ). When the resistant cell line wastransferred from selection medium ontonormal MS medium, its free prolinecontent declined to the normal level insix days (Fig. 7). It suggested that freeproline accumulation was related to osmotic regulation and was the major wayto prevent water loss of the resistant cellline under water stress. Toeal aminoacid analysis revealed that methioninecontent of the regenerated plantletleaves derived from hydroxyproline-resistant protoplastS was twice as high asthe original type (Table 1 ).