| 拟南芥中一个类似钙调素蛋白的基因结构及其缺磷、缺钾诱导表达 |
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| 引用本文： | 段瑞君,易可可,吴平.拟南芥中一个类似钙调素蛋白的基因结构及其缺磷、缺钾诱导表达[J].植物生理与分子生物学学报,2005,31(5):520-526. |
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| 作者姓名： | 段瑞君 易可可 吴平 |
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| 作者单位： | 1. 浙江大学生命科学学院植物生理学与生物化学国家重点实验室,杭州,310029;青海大学生物系,西宁,810016
2. 浙江大学生命科学学院植物生理学与生物化学国家重点实验室,杭州,310029 |
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| 摘 要： | 通过拟南芥芯片杂交分析发现推测的钙调素基因(GenBank accession No.Atlg76650)与低磷胁迫有关.对该基因的结构研究确认了该基因编码一个含有三个EF-hand结构域的类似钙调素的蛋白,Northern检测表明该基因在缺钾、缺磷条件下诱导表达,但在缺氮、高盐等胁迫条件下不受影响.经RT-PCR和启动子融合GUS转基因植株的组织化学染色分析,表明该基因在拟南芥中为全株表达,但各器官中表达丰度不尽相同.
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| 关 键 词： | 拟南芥 类似钙调素蛋白的基因 磷或钾缺乏 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2005-08-03 |
The Structure and Phosphorus or Potassium Deficiency Induced Expression of a Calmodulin-like Protein Gene in Arabidopsis |
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| DUAN Rui-Jun,YI Ke-Ke,WU Ping.The Structure and Phosphorus or Potassium Deficiency Induced Expression of a Calmodulin-like Protein Gene in Arabidopsis[J].Journal Of Plant Physiology and Molecular Biology,2005,31(5):520-526. |
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| Authors: | DUAN Rui-Jun YI Ke-Ke WU Ping |
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| Institution: | 1.The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 2.Department of Biology, Qinghai University, Qinghai 810016, China |
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| Abstract: | According to our previous microarray analysis, we found a putative calmodulin gene related to Pi deficiency and designated AtPsiCaM (Arabidopsis Pi-starvation-induced CaM). Results of structural analysis indicate that AtPsiCaM has three conserved EF-hands motif and belongs to calmodulin-like proteins family (Figs. 1-3). Northern blot analysis revealed that this gene could be induced by potassium and phosphate deficiency and not by potassium deficiency or high salinity (Fig. 4). The results of RT-PCR and GUS histochemical staining assays of the AtPsiCaM promoter::GUS transgenic plants showed that this gene can be expressed in all tissues to different expression levels (Figs. 5, 6). |
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| Keywords: | Arabidopsis thaliana calmodulin-like gene phosphorus or potassium deficiency |
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