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铅污染下芦苇体内铅的分布和铅胁迫相关蛋白
引用本文:江行玉,赵可夫.铅污染下芦苇体内铅的分布和铅胁迫相关蛋白[J].植物生理与分子生物学学报,2002,28(3):169-174.
作者姓名:江行玉  赵可夫
作者单位:山东师范大学逆境植物研究所,济南,250014
基金项目:国家重点基础研究发展规划项目 (G19990 1170 0 ),环境水化学国家重点实验室开放基金资助
摘    要:用Pb(NO3 ) 2 10mmol/L处理芦苇幼苗 7d后 ,检测铅在其不同器官、细胞不同区隔和不同化学状态之间的分布以及铅胁迫相关蛋白。结果显示 ,受铅污染后芦苇幼苗根、地下茎、茎和叶片的铅含量增加 ,它们的大小顺序为根 >地下茎 >茎 >叶片。细胞不同部分铅含量的大小顺序为细胞间隙 >细胞壁 >液泡 >细胞质。在根部和叶片中 ,均以活性较低的醋酸可提取态铅和盐酸可提取态铅占优势。在铅胁迫下 ,从芦苇根和叶片中检测出一些铅结合蛋白。另外 ,铅除诱导芦苇合成一种新的不结合铅的蛋白质外 ,还导致一种分子量大约为 72 0 0 0的蛋白质消失。芦苇抗铅胁迫有以下几种机制 :根部比地上部分积累较多的铅 ;铅在体内形成难溶性化合物 ;铅沉淀在质外体内 ;形成铅结合蛋白质和诱导蛋白

关 键 词:芦苇  铅污染  器官间分布  亚细胞分布  化学状态  铅胁迫相关蛋白
修稿时间:2001年4月19日

The Distribution of Pb and Pb-stressed Relative Proteins in Pb-polluted Phragmites australis Seedlings
JIANG Xing-Yu,ZHAO Ke-Fu.The Distribution of Pb and Pb-stressed Relative Proteins in Pb-polluted Phragmites australis Seedlings[J].Journal Of Plant Physiology and Molecular Biology,2002,28(3):169-174.
Authors:JIANG Xing-Yu  ZHAO Ke-Fu
Abstract:The Pb distributions of different organs and subcellular compartments, the relative abundance of different chemical forms of Pb as well as Pb-stressed relative proteins in Phragmites australis seedlings were determined after the seedlings were exposed to Pb(NO 3) 2 10 mmol/L solution for 7 d. The atomic absorption spectrophotometric analysis revealed that the levels of Pb in roots, rhizomes, stems and leaves of the seedlings increased significantly in decreasing order in the presence of Pb 2 in the culture solution (Table 1). In order to study Pb compartmentalization within the root cells of P. australis seedlings polluted by Pb, transmission electron microscopy and X-ray energy spectrum microanalysis had been applied to measure the levels of Pb in various parts of root cortex cells of P. australis seedlings. The results showed that Pb distribution sequence is: intercellular space>cell wall>vacuole>cytoplasm (Table 2). The study of Pb forms using sequential extraction method showed that the extraction ratio of Pb extracted respectively was different from different extraction media, of which less active HAc-extracted and HCl extracted fractions were the main forms in roots and in leaves (Table 3). By gel filtration, some Pb-binding complexes were formed in roots and leaves of P. australis seedlings polluted by Pb. In addition, Pb stress also induced a new protein with a mol. wt. of 30 000 and led to the disappearance of a protein with a mol. wt. of 72 000 (Fig.1). All results suggest that the possible defense mechanism in P. australis involves Pb accumulation in root; formation of insoluble complexes; concentration in apoplast and vacuole by compartmentalization; the binding with Pb of specific proteins. In addition, P. australis can synthesize a new protein which might function to protect P. australis cell from Pb stress.
Keywords:Phragmites australis  Pb pollution  organ distribution of Pb  subcellular distribution of Pb  chemical forms of Pb  Pb-stressed relative proteins
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