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Clotrimazole对菠菜叶绿体光合磷酸化和电子传递的抑制作用
引用本文:魏家绵,李德耀,张鸿龙.Clotrimazole对菠菜叶绿体光合磷酸化和电子传递的抑制作用[J].植物生理与分子生物学学报,1988(3).
作者姓名:魏家绵  李德耀  张鸿龙
作者单位:中国科学院上海植物生理研究所,中国科学院上海植物生理研究所,中国科学院上海药物研究所 上海 200032,上海 200032,上海 200031
摘    要:10μmol/的clotrimazole不仅抑制光合磷酸化活力,而且抑制各种类型的电子传递,是一个典型的电子传递抑制剂。经过它对叶绿体放氧,荧光和毫秒延迟发光影响的比较研究表明:clotri—mazole在光合电子传递链上的作用部位在Q与PQ之间,即与DGMU的作用部位相同或相近。

关 键 词:光合磷酸化  电子传递抑制剂  光系统Ⅰ  延迟发光

The Inhibitory Effect of Clotrimazole on Photophosphorylation and Electron Transport in Chloroplasts of Spinach
WEI Jia-Mian,LI De-Yao.The Inhibitory Effect of Clotrimazole on Photophosphorylation and Electron Transport in Chloroplasts of Spinach[J].Journal Of Plant Physiology and Molecular Biology,1988(3).
Authors:WEI Jia-Mian  LI De-Yao
Institution:WEI Jia-Mian,LI De-Yao Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032 ZHANG Hong-Long Shanghai Institute of Pharmacology,Academia Sinica,Shanghai 200031
Abstract:The inhibitory effect of clotrimazole on photophosphorylation and electron transport was studied. The results showed that clotrima- zole inhibited not only the rate of photophospho- rylation, but also the rate of electron transport and O_2 evolution in chloroplasts. The effects of clotrimazole on ms-delayed light emission and fluorescence of chloroplasts were very similar to that of DCMU, but quite different from that of NH_2OH and DBMIB. Basing on these, it has been deduced that the action site of clotrimazole in electron trans- port chain is the same as or near that of DCMU. Therefore, clotrimazole is a new inhibitor of electron transport, which may find special use in the study of bioenergetics.
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