杏花开放过程中超弱发光和ATP及活性氧含量的变化

以巴旦水杏 (Prunus armeniaca,Ameniacavulgaris Lam.)为试验材料,研究了杏花开放过程中花朵的超弱发光(ultraweak 1 uminescence,UWL)变化及其与ATP含量、呼吸速率、活性氧产生速率等的关系.结果表明,杏花开放的前半期(盛花期以前),超氧自由基产生速率与丙二醛含量变化平缓,UWL、ATP含量、呼吸速率及蛋白质含量呈上升趋势.盛花期过后,呼吸速率、蛋白质和ATP含量迅速降低,UWL强度缓慢下降,而超氧自由基的产生速率与丙二醛含量迅速升高.分析后认为,杏花的超微弱发光与以ATP含量为标志的能量水平密切相关,同时也受体内活性氧及其它物质代谢的影响;盛花期以前ATP可能是导致UWL升高的主要因素,盛花期过后,活性氧可能对花体的UWL变化起了部分的调节作用.

Changes in Ultraweak Luminescence,ATP and Active Oxygen Contents During Apricot Florescence

Badan Shuixing apricot (Amenia cavulgaris Lam.) was used to study the changes in UWL intensity and its relationship to ATP content, respiration rate and active oxygen production rate during florescence of apricot. The results indicated that the production rate of active oxygen was smooth before the peak season of blossom, the UWL intensity and ATP content were both rise and the respiration rate also showed the trend of rise mainly at the same time. After the peak season of blossom, the respiration rate and ATP content declined rapidly while the UWL intensity went down slowly, but the active oxygen production rate increased rapidly. The protein content increased at first and then decreased during the florescence of apricot. The change in MDA content was similar to the change in active oxygen production rate, which rose rapidly after the peak season of blossom. It was concluded that the UWL was closely related to the energy level symbolized by ATP content and was affected by active oxygen metabolism in the apricot flower. The ATP content was the main factor to lead to the rise of UWL before the full bloom stage while the action of active oxygen on UWL appeared just after the peak season of blossom.