盐胁迫下盐芥渗透调节物质的积累及其渗透调节作用

用含有NaCl0、50、100、200、300、400mmol/L的Hoagland培养液处理盐芥幼苗一定时间后,分别测定其根和叶含水量、渗透势、几种无机和有机渗透调节物质含量,并计算了渗透调节物质在不同条件下的计算渗透势值(COP).结果表明:随盐处理浓度的增加,盐芥根和叶的含水量和渗透势逐渐降低;Na 和Cl-是根和叶积累的无机渗透调节物质;SS、OA和FAA是根积累的有机渗透调节物质,Pro是叶和根积累的有机渗透调节物质.Na X-ray微区分析表明液泡是积累Na 的主要部位.

Osmotica Accumulation and Its Role in Osmotic Adjustment in Thellungiella halophila Under Salt Stress

Thellungiella halophila was treated with different concentrations of NaCl (0, 50, 100, 200, 300 and 400 mmol/L). Water content, osmotic potential, various organic and inorganic osmotica contents in the leaves and roots were determined and the calculated osmotic potential (COP) of osmotica in the osmotic adjustment was calculated (Figs.1-10 and Table 1). The result showed that water content, osmotic potential decreased with the increase of salinity; Na(+) and Cl(-) were the accumulated inorganic osmotica in roots and leaves; soluble sugar, organic acid and free amino acid were the accumulated organic osmotica in roots; and proline was the accumulated organic osmotica in both root and leaf. Na(+) X-ray microanalysis indicated that vacuole in the cell was the main organelle of accumulation toxic ions (Table 2).