水稻细胞质雄性不育系幼穗发育过程中多胺与乙烯的关系初探(英文 )

利用ＨＰＬＣ和ＧＣ分别测定了水稻细胞质雄性不育系及其保持系幼穗多胺（ 腐胺，亚精胺和精胺） 含量和乙烯释放速率，并研究了外施多胺合成抑制剂ＭＧＢＧ 和乙烯前体ＡＣＣ生成抑制剂ＡＶＧ 对两系幼穗多胺含量和乙烯释放速率以及花粉育性的影响。结果表明， 不育系幼穗乙烯释放速率显著高于其保持系幼穗， 外施ＡＶＧ 引起两系幼穗乙烯释放速率下降，并使不育系花粉育性得以部分恢复； 不育系幼穗多胺含量显著低于保持系幼穗， 外施ＭＧＢＧ 使两系幼穗Ｓｐｄ 和Ｓｐｍ 含量下降， 并使保持系花粉育性降低。外施ＡＶＧ 抑制乙烯释放，促进多胺合成；而外施ＭＧＢＧ 抑制Ｓｐｄ和Ｓｐｍ 合成， 却促进乙烯的释放； 而且，乙烯释放速率与多胺（精胺和亚精胺） 含量呈显著负相关。提示在水稻ＣＭＳ 系及其保持系幼穗发育过程中乙烯与多胺（ 精胺和亚精胺） 的生物合成竞争ＳＡＭ。

Preliminary Study on the Relationship Between Polyamine and Ethylene During Panicle Development in Cytoplasmic Male Sterile Rice

By using HPLC and GC, polyamines(Put, Spd and Spm) contents and ethylene release rate(ERR) were measured in the panicles of CMS line and its maintainer line of rice. The effects of MGBG, an inhibitor of biosynthesis of Spd and Spm, and AVG, an inhibitor of ACC biosynthesis, on PA content and ERR in the panicles and the fertility of pollen in the two lines were studied,too. The results showed that ERR in the panicles of CMS line was higher than in its maintainer line.Treatment with AVG decreased ERR in the panicles of both lines, and restored partly pollen fertility in CMS line. PA content in the panicles of CMS line was lower than these in those of its maintainer line. Treatment with MGBG decreased Spd and Spm levels in both lines,and pollen fertility in maintainer line. AVG inhibited ethylene production and promoted PA synthesis. MGBG inhibited PA synthesis and promoted ethylene production. Moreover, ethylene production was negatively correlated with the content of Spd Spm in the panicles of both lines. It is shown that PA(Spd and Spm) and ethylene biosynthesis pathways compete for SAM during the development of panicle of CMS line and its maintainer line in rice.