高产人参寡糖素培养细胞克隆系的诱变筛选

紫外辐射能显著地降低人参培养细胞单细胞克隆的植板率。当紫外辐射悬浮细胞３０ｓ后，细胞克隆的植林率是对照组的２１．４３％。细胞克隆平板培养６０ｄ，挑取克隆连续转移培养３次，共获得克隆系１２２株。对所有克隆系进行变异分析并经１０代连续继代培养，从中筛选到一株稳定的高产寡糖素克隆系ＰＧＵＡ－０８，而且它的过氧化物酶同工酶谱特征也保持稳定。克隆系ＰＧＵＡ－０８的生长速率为０．５３７ｇＤＷＬ－１ｄ－１，是亲本的１．４６倍，寡糖素含量为１７．１６％ＤＷ，是亲本的１．８１倍，寡糖素产率为２．７６４ｇ／Ｌ，是亲本的２．６２倍。

Selection of Variants with High Yield of Oligosaccharin from Panax ginseng Cultured Cells

Cultured cells of Panax ginseng were found to be highly sensitive to UV--irradiation,which could warkedly decrease the plating efficiency of single cell cloning of P. ginseng cultured cells.After cultured cells were treated with UV--irradiation for 30 s, the plating efficiency was 21. 4% of the control (Fig. 1 ). Cell growth rate and oligosaccharin content of all 122 clone lines obtained from 165 clone lines through 3 times transplantution culture, chosen after 60 d of plating culture, were measured (Table 1, Fig. 2and 3 ). Basing on the stability of clone lines obtained through 10 generations successive subculture, a stable clone line PGUA--08 with high yield of oligosaccharin had been selected (Fig.4 ). Moreover, the isozyme patterns of peroxidases of clone line PGUA-08 maintained almost unchanged during successive subculture (Fig. 5 ). The growth rate of clone line PGUA-08 was 0. 537 g DW-1 L-1 d-1, and was46% higher than that of its parent,and its oligosaccharin content and yieldwere 17. 16% DW and 2. 764 g/L,respectively, 1-. 81 and 2. 62 times that of its parent, respectively (Table 2).