Comparison of the karyotypes ofPsathyrostachys juncea andP. huashanica (Poaceae) studied by banding techniques |
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Authors: | Dr Ib Linde-Laursen Dr Roland von Bothmer |
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Institution: | 1. Agricultural Research Department, Ris? National Laboratory, DK-4000, Roskilde, Denmark 2. Department of Crop Genetics and Breeding, Swedish University of Agricultural Sciences, S-268 00, Sval?v, Sweden
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Abstract: | The karyotypes ofP. juncea (Elymus junceus) andP. huashanica (both outbreeders) were investigated by Feulgen-staining and by C-, N-, and Agbanding, based on a single plant in cach case.
Both species have 2n=2x=14 and large chromosomes, possibly a generic character. The karyotype ofP. juncea has 8 metacentrics and 6 SAT-chromosomes with minute, heterochromatic satellites while that ofP. huashanica has 9 metacentrics and 5 SAT-chromosomes only, 2 of which with small, heterochromatic satellites. The C-banding patterns
ofP. juncea chromosomes comprise from one to five, mostly small, bands at distal, and terminal positions, while those ofP. huashanica chromosomes are characterized by large telomeric bands in most arms. Banding patterns and chromosome morphology allow identification
of the homologues of the seven chromosome pairs inP. juncea, but of two pairs inP. huashanica only. The patterns of both taxa are polymorphic, supporting that both taxa are outbreeders. The karyotypic characters suggest
thatP. juncea is more closely related toP. fragilis than either is toP. huashanica. N-banding stains weakly. Silver nitrate staining demonstrates that nucleolus organizers of both species have different nucleolus
forming capacities. The presence of micronucleoli suggests that both species have an extra unidentified chromosome with nucleolus
forming capacity. |
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Keywords: | Angiosperms Poaceae Psathyrostachys juncea P huashanica — Chromosomes Giemsa C-banding patterns chromosome polymorphism nucleoli |
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