Isotope dilution mass spectrometry for the quantification of sulfane sulfurs |
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| Institution: | 1. Department of Chemistry, Washington State University, Pullman, WA 99164, USA;2. Research Institute of Science and Technology, Central China Normal University, Wuhan 430079, China;3. Department of Pharmaceutical Sciences, Washington State University, Pullman, WA 99164, USA;4. Center for Reproductive Biology, Molecular Biology and Genomics Core, Washington State University, Pullman, WA 99164, USA;1. Dip. di Matematica e Applicazioni, Università “Federico II”, Complesso Universitario M. S. Angelo, Via Cintia, 80126 Napoli, Italy;2. INFN, Sezione di Napoli, Complesso Universitario M. S. Angelo, Via Cintia, 80126 Napoli, Italy;3. Centro de Energías Alternativas y Ambiente, Escuela Superior Politécnica del Chimborazo, Riobamba, Ecuador;4. Dip. di Matematica ed Informatica, Universitá della Calabria, Arcavacata, Rende, Italy;1. Department of Chemistry & Biochemistry, University of Windsor, Windsor, Ontario N9B 3P4, Canada;2. Department of Medical Biochemistry, Akdeniz University Medical School, 07070 Antalya, Turkey;1. William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK;2. School of Biomedical Engineering & Imaging Sciences, King''s College London, London, UK |
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| Abstract: | Sulfane sulfurs are one type of important reactive sulfur species. These molecules have unique reactivity that allows them to attach reversibly to other sulfur atoms and exhibit regulatory effects in diverse biological systems. Recent studies have suggested that sulfane sulfurs are involved in signal transduction processes regulated by hydrogen sulfide (H2S). Accurate and reliable measurements of sulfane sulfurs in biological samples are thus needed to reveal their production and mechanisms of actions. Herein we report a convenient and accurate method for the determination of sulfane sulfur concentrations. The method employs a triphenylphosphine derivative (P2) to capture sulfane sulfurs as a stable phosphine sulfide product, PS2. The concentration of PS2 was then determined by isotope dilution mass spectrometry, using a 13C3-labeled phosphine sulfide, PS1, as the internal standard. The specificity and efficiency of the method were proven by model reactions. It was also applied to the measurement of sulfane sulfurs in mouse tissues including brain, kidney, lung, liver, heart, spleen, and blood. |
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| Keywords: | Reactive sulfur species Isotope dilution assay Sulfane sulfur Mass Free radicals |
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