Institution: | Rijk Zwaan Breeding BV, Fijnaart, The Netherlands; Max F. Perutz Laboratories, Department of Plant Molecular Biology, Vienna University, Vienna, Austria; Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, PA, USA; School of Life Sciences, Institute of Plant Biology, Fudan University, Shanghai, China; Laboratory of Genetics, Wageningen University, Wageningen, the Netherlands |
Abstract: | Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non-recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity . Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome. |