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Evaluation of cDNA Libraries from Different Developmental Stages of Schistosoma mansoni for Production of Expressed Sequence Tags (ESTs)
Authors:Franco  Gloria R; Rabelo  Elida M L; Azevedo  Vasco; Pena  Heloisa B; Ortega  J Miguel; Santos  Tulio M; Meira  Wendell S F; Rodrigues  Neuza A; Dias  Carlos M M; Harrop  Richard; Wilson  Alan; Saber  Mohamed; Abdel-Hamid  Hannan; Faria  Michelyne S C; Margutti  Maria Elizabeth B; Parra  Jucara C; Pena  Seergio D J
Institution:1Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais Belo Horizonte, Brazil 31270-010
2Departamento de Parasitologia, Universidade Federal de Minas Gerais Belo Horizonte, Brazil 31270-010
3Departamento de Biologia Geral, Universidade Federal de Minas Gerais Belo Horizonte, Brazil 31270-010
4Departamento de Microbiologia, Universidade Federal de Minas Gerais Belo Horizonte, Brazil 31270-010
5Department of Biology, University of York York, Y015DD, UK
6Theodore Bilharz Research Institute Cairo, 12411, Egypt
7Centro de Pesquisas René Rachou Belo Horizonte, Brazil 30190-002
Abstract:A comparative study of the gene expression profile in differentdevelopmental stages of Schistosoma mansoni has been initiatedbased on the expressed sequence tag(EST) approach. A total of1401 ESTs were generated from seven different cDNA librariesconstructed from four distinct stages of the parasite life cycle.The libraries were first evaluated for their quality for a large-scalecDNA sequencing program. Most of them were shown to have lessthan 20% useless clones and more than 50% new genes. The redundancyof each library was also analyzed, showing that one adult wormcDNA library was composed of a small number of highly frequentgenes. When comparing ESTs from distinct libraries, we coulddetect that most genes were present only in a single library,but others were expressed in more than one developmental stageand may represent housekeeping genes in the parasite. When consideringonly once the genes present in more than one library, a totalof 466 unique genes were obtained, corresponding to 427 newS. mansoni genes. From the total of unique genes, 20.2% wereidentified based on homology with genes from other organisms,8.3% matched S. mansoni characterized genes and 71.5% representunknown genes.
Keywords:Schistosoma mansoni  developmental stages  cDNA sequencing analysis  expressed sequence tags
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