Generation of 7137 non-redundant expressed sequence tags from a legume, Lotus japonicus. |
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Authors: | E Asamizu Y Nakamura S Sato S Tabata |
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Institution: | Kazusa DNA Research Institute, Kisarazu, Chiba, Japan. |
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Abstract: | For comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 22,983 5' end expressed sequence tags (ESTs) were accumulated from normalized and size-selected cDNA libraries constructed from young (2 weeks old) plants. The EST sequences were clustered into 7137 non-redundant groups. Similarity search against public non-redundant protein database indicated that 3302 groups showed similarity to genes of known function, 1143 groups to hypothetical genes, and 2692 were novel sequences. Homologues of 5 nodule-specific genes which have been reported in other legume species were contained in the collected ESTs, suggesting that the EST source generated in this study will become a useful tool for identification of genes related to legume-specific biological processes. The sequence data of individual ESTs are available at the web site: http://www.kazusa.or.jp/en/plant/lotus/EST/. |
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Keywords: | Legume Lotus japonicus cDNA EST |
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