Mapping of quantitative trait locus related to submergence tolerance in rice with aid of chromosome walking. |
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Authors: | W Kamolsukyunyong V Ruanjaichon M Siangliw S Kawasaki T Sasaki A Vanavichit S Tragoonrung |
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Institution: | National Center for Genetic Engineering and Biotechnology, Kasetsart University, Nakorn Pathom, Thailand. wintai@dna.kps.ku.ac.th |
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Abstract: | The major QTL for submergence tolerance was locate in the 5.9 cM interval between flanking RFLP markers. To narrow down this region, a physical map was constructed using YAC and BAC clones. A 400-kb YAC was identified in this region and later its end fragments were used to screen a rice BAC library. Through chromosome walking, 24 positive BAC clones formed two contigs around linked-RFLP markers, R1164 and RZ698. Using one YAC end, six BAC ends and three RFLP markers, a fine-scale map was constructed of the 6.8-cM interval of S10709-RZ698 on rice chromosome 9. The submergence tolerance and related trait were located in a small, well-defined region around BAC-end marker 180D1R and RFLP marker R1164. The physical-to-map distance ratio in this region is as small as 172.5 kb/cM, showing that this region is a hot spot for recombination in the rice genome. |
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Keywords: | rice (Oryza sativa L ) submergence tolerance positional cloning physical mapping bacterial artificial chromosome (BAC) yeast artificial chromosome (YAC) |
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