Production of cyclodextrin glycosyltransferase by alkaliphilic <Emphasis Type="Italic">Bacillus circulans</Emphasis> in submerged and solid-state cultivation |
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Authors: | Flávia Santos Twardowski Pinto Simone Hickmann Flôres Marco Antônio Záchia Ayub Plinho Francisco Hertz |
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Institution: | 1.Instituto de Ciência e Tecnologia de Alimentos,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil |
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Abstract: | Cyclodextrin glycosyltransferase (CGTase; E.C. 2.4.1.19) is an industrially important enzyme, which is used to produce cyclodextrins
(CDs). In this research, we report the use of experimental factorial design to find the best conditions of pH and temperature
for CGTase production by Bacillus circulans var. alkalophilus. The optimized calculated values for the tested variables were, respectively, pH 9.7 and temperature 36oC, with a CGTase activity of 615 U mL−1. The CGTase production was further studied with the optimized process parameters on submerged cultivations (SC) and solid-state
cultivations (SSC) using soybean industrial fibrous residue (SIFR). The maximum CGTase activity obtained on SC was 1,155 U mL−1 under aerobic conditions. Cell growth and CGTase synthesis in SSC using SIFR as substrate was excellent, with CGTase activity
of 32,776 U g(SIFR) −1. These results strongly support the use of SIFR for CGTase production since it is a non-expensive residue. |
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Keywords: | Bacillus circulans Submerged cultivation Solid-state cultivation Cyclodextrin glycosyltransferase |
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