Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of <Emphasis Type="Italic">Streptococcus zooepidemicus</Emphasis> |
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Authors: | Long Liu Guocheng Du Jian Chen Miao Wang Jun Sun |
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Institution: | (1) School of Biotechnology, Jiangnan University, 214122 Wuxi, China;(2) Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122 Wuxi, China;(3) State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China;(4) School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China;(5) Institute of Information Technology, Jiangnan University, 214122 Wuxi, China |
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Abstract: | Three different dissolved oxygen (DO) control approaches were proposed to improve hyaluronic acid (HA) production: a three-stage
agitation speed control approach, a two-stage DO control approach, and an oxygen vector perfluorodecalin (PFC) applied approach.
In the three-stage agitation speed control approach, agitation speed was 200 rpm during 0–8 h, 400 rpm during 8–12 h, and
600 rpm during 12–20 h. In the two-stage DO control strategy, DO was controlled at above 10% during 0–8 h and at 5% during
8–20 h. In the PFC applied approach, PFC (3% v/v) was added at 8 h. HA production reached 5.5 g/L in the three-stage agitation
speed control culture model, and 6.3 g/L in two-stage DO control culture model, and 6.6 g/L in the PFC applied culture model.
Compared with the other two DO control approaches, the PFC applied approach had a lower shear stress and thus a higher HA
production was achieved. |
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Keywords: | Hyaluronic acid Streptococcus zooepidemicus Oxygen mass transfer Two-stage Oxygen vector |
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