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Optimization of temperature and initial pH and kinetic analysis of tartaric acid production by Gluconobacter suboxydans |
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| Authors: | K Chandrashekar P Arthur Felse T Panda |
| Institution: | (1) School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-742, South Korea;(2) Department of Chemical System Engineering, College of Science and Technology, Hongik University, Jochiwon, Chungnam, 339-701, South Korea;(3) Department of Molecular Science and Technology, Ajou University, Suwon, 442-749, South Korea;(4) Department of Life Science and Biotechnology, Faculty of Life &; Environmental Science, Shimane University, Matsue 690-8504, Japan; |
| Abstract: | Response surface methodology was applied for the simultaneous optimization of initial pH and temperature of fermentation for the production of tartaric acid by Gluconobacter suboxydans. A temperature of 32.8v°C and an initial pH of 6.05 was found to be most suitable. Kinetic analysis for the growth of Gluconobacter suboxydans and product formation was done and a logistic model was used to describe the growth of Gluconobacter suboxydans while the Leudeking-Piret model explained the product formation. Parameters of the model were evaluated and tartaric acid formation was found to be non-growth associated. |
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